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Therapeutic targeting of endothelial calcium signaling accelerates the resolution of lung injury

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Udgivet i:Signal Transduction and Targeted Therapy vol. 10, no. 1 (2025), p. 375-396
Hovedforfatter: Chan, Wan Ching
Andre forfattere: Kwok, Man Long, Qu, Xinyan, Abdelkarim, Hazem, Le, Jonathan, Yang, Deying, Banerjee, Avik, Zhao, Shuangping, Class, Jacob, Gonzalez, Marlen, Hailemeskel, Harry, Singh, Raman Ghotra, Gallardo-Macias, Ricardo, Gurvich, Vadim J., Maienschein-Cline, Mark, Lindeblad, Matthew, Kabirov, Kasim, Lyubimov, Alexander V., Belvitch, Patrick, Richner, Justin, Gaponenko, Vadim, Komarova, Yulia A.
Udgivet:
Nature Publishing Group
Fag:
Morbidity
Calcium signalling
Endoplasmic reticulum
Severe acute respiratory syndrome coronavirus 2
Mortality
Sepsis
Physicochemical properties
Therapeutic targets
Respiratory distress syndrome
Amino acids
NF-κB protein
Calcium (intracellular)
Peptides
NF-AT protein
Drug development
Kinases
Nuclear magnetic resonance > NMR
Proteins
COVID-19
Calcium (reticular)
Immune response
Hypoxemia
Edema
Pneumonia
Pathophysiology
Lung diseases
Design
Endothelial cells
Inositol trisphosphate
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Resumen:Acute respiratory distress syndrome (ARDS) is a severe pulmonary disease characterized by acute, noncardiogenic pulmonary edema and hypoxemia leading to respiratory failure. It is induced by a diverse array of etiologies, including recent SARS-CoV-2 infection. The current standard of care for ARDS remains predominantly supportive, underscoring the urgent need for targeted pharmacological interventions. To address this critical gap, we developed an inhibitor of the microtubule accessory factor end-binding protein 3 (EB3), a key mediator of pathological calcium signaling in endothelial cells. During injury, EB3 facilitates inositol 1,4,5-trisphosphate receptor 3 (IP3R3) clustering on the endoplasmic reticulum membrane, activating widespread calcium release from intracellular stores and leading to endothelial barrier disruption. Using nuclear magnetic resonance (NMR)-guided approaches, we designed and optimized a synthetic EB3 inhibitor, termed vascular therapeutics (VT)-109, with enhanced physicochemical properties. We evaluated the therapeutic potential of VT-109 across a wide range of preclinical models in which pathogenic insults target epithelial or endothelial barriers. Treatment with VT-109 promptly restored the tissue‒fluid balance in the injured lung by inducing the reannealing of VE-cadherin junctions and restoring the endothelial barrier. In addition to vascular protection, VT-109 improved lung architecture and function, normalized immune responses, and significantly reduced both morbidity and mortality in ARDS models. At the molecular level, VT-109 blocks inflammatory NFAT and NFκB signaling while concurrently activating FOXM1-dependent endothelial regeneration. These findings support EB3 inhibition as a promising therapeutic strategy for ARDS and highlight VT-109 as a versatile drug candidate capable of addressing the multifaceted pathophysiology of this disease.
ISSN:2095-9907
2059-3635
DOI:10.1038/s41392-025-02461-y
Fuente:Health & Medical Collection