Temporal sampling of root exudates using coated blade-SPME for decoding plant–pathogen interactions
Guardado en:
| 发表在: | Frontiers in Plant Science vol. 16 (Sep 2025), p. 1616881-1616891 |
|---|---|
| 主要作者: | |
| 其他作者: | , |
| 出版: |
Frontiers Media SA
|
| 主题: | |
| 在线阅读: | Citation/Abstract Full Text Full Text - PDF |
| 标签: |
没有标签, 成为第一个标记此记录!
|
MARC
| LEADER | 00000nab a2200000uu 4500 | ||
|---|---|---|---|
| 001 | 3273798032 | ||
| 003 | UK-CbPIL | ||
| 022 | |a 1664-462X | ||
| 024 | 7 | |a 10.3389/fpls.2025.1616881 |2 doi | |
| 035 | |a 3273798032 | ||
| 045 | 2 | |b d20250901 |b d20250930 | |
| 100 | 1 | |a Halane, Hodan |u London Research and Development Centre, Agriculture and Agri-Food Canada, London, ON, Canada, Department of Biology, University of Western Ontario, London, ON, Canada | |
| 245 | 1 | |a Temporal sampling of root exudates using coated blade-SPME for decoding plant–pathogen interactions | |
| 260 | |b Frontiers Media SA |c Sep 2025 | ||
| 513 | |a Journal Article | ||
| 520 | 3 | |a Root exudates shape the rhizosphere and mediate plant–microbe interactions, yet traditional sampling techniques often disturb the natural environment. Here, we present the use of coated blade-solid-phase microextraction (CB-SPME) method for passive, non-invasive in situ temporal metabolomic profiling of pea ( Pisum sativum ) root exudates during infection by the soil-borne pathogen Aphanomyces euteiches. In comparison to previously established extraction techniques, CB-SPME delivered lower absolute recovery but superior reproducibility while maintaining sensitivity. This non-destructive approach preserves rhizosphere integrity, enabling continuous monitoring of dynamic metabolite fluctuations and offers new insights into how root exudate influences plant–microbiome interactions. | |
| 653 | |a Pathogens | ||
| 653 | |a Soil ecology | ||
| 653 | |a Sampling techniques | ||
| 653 | |a Sampling methods | ||
| 653 | |a Metabolomics | ||
| 653 | |a Seeds | ||
| 653 | |a Rhizosphere | ||
| 653 | |a Natural environment | ||
| 653 | |a Exudates | ||
| 653 | |a Metabolites | ||
| 653 | |a Methods | ||
| 653 | |a Sampling | ||
| 653 | |a Hydroponics | ||
| 653 | |a Research & development--R&D | ||
| 653 | |a Roots | ||
| 653 | |a Exudation | ||
| 653 | |a Solid phases | ||
| 653 | |a Solid phase methods | ||
| 653 | |a Microbiomes | ||
| 653 | |a Environmental | ||
| 700 | 1 | |a McDowell, Tim |u London Research and Development Centre, Agriculture and Agri-Food Canada, London, ON, Canada | |
| 700 | 1 | |a Dhaubhadel, Sangeeta |u London Research and Development Centre, Agriculture and Agri-Food Canada, London, ON, Canada, Department of Biology, University of Western Ontario, London, ON, Canada | |
| 773 | 0 | |t Frontiers in Plant Science |g vol. 16 (Sep 2025), p. 1616881-1616891 | |
| 786 | 0 | |d ProQuest |t Agriculture Science Database | |
| 856 | 4 | 1 | |3 Citation/Abstract |u https://www.proquest.com/docview/3273798032/abstract/embedded/L8HZQI7Z43R0LA5T?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text |u https://www.proquest.com/docview/3273798032/fulltext/embedded/L8HZQI7Z43R0LA5T?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text - PDF |u https://www.proquest.com/docview/3273798032/fulltextPDF/embedded/L8HZQI7Z43R0LA5T?source=fedsrch |