Delaying Candidatus Liberibacter asiaticus infection of citrus trees through use of individual protective covers and systemic delivery of oxytetracycline
Guardat en:
| Publicat a: | Frontiers in Plant Science vol. 16 (Oct 2025), p. 1671217-1671238 |
|---|---|
| Autor principal: | |
| Altres autors: | , , , , |
| Publicat: |
Frontiers Media SA
|
| Matèries: | |
| Accés en línia: | Citation/Abstract Full Text Full Text - PDF |
| Etiquetes: |
Sense etiquetes, Sigues el primer a etiquetar aquest registre!
|
MARC
| LEADER | 00000nab a2200000uu 4500 | ||
|---|---|---|---|
| 001 | 3273798089 | ||
| 003 | UK-CbPIL | ||
| 022 | |a 1664-462X | ||
| 024 | 7 | |a 10.3389/fpls.2025.1671217 |2 doi | |
| 035 | |a 3273798089 | ||
| 045 | 2 | |b d20251001 |b d20251031 | |
| 100 | 1 | |a Tardivo, Caroline |u Horticultural Sciences Department, University of Florida/IFAS, Southwest Florida Research and Education Center, Immokalee, FL, United States | |
| 245 | 1 | |a Delaying Candidatus Liberibacter asiaticus infection of citrus trees through use of individual protective covers and systemic delivery of oxytetracycline | |
| 260 | |b Frontiers Media SA |c Oct 2025 | ||
| 513 | |a Journal Article | ||
| 520 | 3 | |a Huanglongbing (HLB), or citrus greening, remains one of the most destructive diseases affecting citrus production globally. Associated with the phloem-limited bacterium Candidatus Liberibacter asiaticus ( C Las) and vectored by Diaphorina citri , HLB leads to canopy decline, fibrous root loss, and reductions in fruit yield and quality. Recently, the systemic delivery of oxytetracycline (OTC) via trunk injection was approved in Florida as a targeted therapy to reduce C Las titers and improve tree health. In parallel, Individual Protective Covers (IPCs) have been adopted to delay C Las infection in newly planted citrus trees by vector exclusion. This study investigates the combined use of IPCs and trunk injection of OTC for post-IPC therapy. ‘Valencia’ sweet orange trees grafted on US-812 and US-942 rootstocks were planted in December 2020 under HLB-endemic conditions in southwest Florida. IPCs were installed at planting and removed after 18 months. The first OTC injection was performed in May 2023, 10 months after IPC removal. A second injection was performed in May 2024. A 2 × 2 × 2 factorial experimental design evaluated the effects of infection history (early-infected and late-infected), rootstock cultivar (US-812 and US-942), and injection treatment (OTC-injected and non-injected) on tree responses over two consecutive production seasons. In year 1, infection history significantly influenced tree size, fruit yield, total soluble solids (TSS), TSS/titratable acidity ratio, and peel color. Late-infected trees outperformed early-infected trees, regardless of injection treatment and rootstock cultivar. In year 2, OTC-injected trees exhibited significantly higher yields, improved juice quality, and enhanced canopy health regardless of infection history and rootstock cultivar. Fibrous root microbiome analyses based on 16S rRNA sequencing revealed no significant effects of OTC injection on bacterial alpha or beta diversity, with stable community structure observed across treatments and time points. This suggests that targeted vascular delivery of OTC may not cause any major disruption to the root endorhizosphere microbiome. Together, the results from this study demonstrate the efficacy of integrating preventative (use of IPCs) and therapeutic (OTC vascular delivery) strategies for sustainable HLB management while preserving microbial integrity and offering a model for citrus production in parts of the world where HLB is prevalent. | |
| 651 | 4 | |a Florida | |
| 651 | 4 | |a United States--US | |
| 653 | |a Infections | ||
| 653 | |a Community structure | ||
| 653 | |a rRNA 16S | ||
| 653 | |a Crop yield | ||
| 653 | |a Oxytetracycline | ||
| 653 | |a Experimental design | ||
| 653 | |a Citrus fruits | ||
| 653 | |a Citrus greening | ||
| 653 | |a Canopies | ||
| 653 | |a Insecticides | ||
| 653 | |a Plant bacterial diseases | ||
| 653 | |a Fruit trees | ||
| 653 | |a Injection | ||
| 653 | |a Citrus trees | ||
| 653 | |a Plant diseases | ||
| 653 | |a Cultivars | ||
| 653 | |a Fruits | ||
| 653 | |a Bacterial diseases | ||
| 653 | |a Microbiomes | ||
| 653 | |a Bacteria | ||
| 653 | |a Trees | ||
| 653 | |a Microorganisms | ||
| 653 | |a Antibiotics | ||
| 653 | |a Design of experiments | ||
| 653 | |a Rootstocks | ||
| 653 | |a Integrated approach | ||
| 653 | |a Candidatus Liberibacter asiaticus | ||
| 653 | |a Environmental | ||
| 700 | 1 | |a Monus, Brittney |u Department of Soil, Water, and Ecosystem Sciences, University of Florida/IFAS, Southwest Florida Research and Education Center, Immokalee, FL, United States | |
| 700 | 1 | |a Pugina, Gabriel |u Horticultural Sciences Department, University of Florida/IFAS, Southwest Florida Research and Education Center, Immokalee, FL, United States | |
| 700 | 1 | |a Strauss, Sarah L |u Department of Soil, Water, and Ecosystem Sciences, University of Florida/IFAS, Southwest Florida Research and Education Center, Immokalee, FL, United States | |
| 700 | 1 | |a Alferez, Fernando |u Horticultural Sciences Department, University of Florida/IFAS, Southwest Florida Research and Education Center, Immokalee, FL, United States | |
| 700 | 1 | |a Albrecht, Ute |u Horticultural Sciences Department, University of Florida/IFAS, Southwest Florida Research and Education Center, Immokalee, FL, United States | |
| 773 | 0 | |t Frontiers in Plant Science |g vol. 16 (Oct 2025), p. 1671217-1671238 | |
| 786 | 0 | |d ProQuest |t Agriculture Science Database | |
| 856 | 4 | 1 | |3 Citation/Abstract |u https://www.proquest.com/docview/3273798089/abstract/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text |u https://www.proquest.com/docview/3273798089/fulltext/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text - PDF |u https://www.proquest.com/docview/3273798089/fulltextPDF/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |