Identification and expression analysis of calcium-dependent protein kinase family in oat (Avena sativa L.) and their functions in response to saline-alkali stresses

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Izdano u:Frontiers in Plant Science vol. 15 (Oct 2024), p. 1395696-1395718
Glavni autor: Li, Ya-nan
Daljnji autori: Lei, Chunyan, Yang, Qian, Yu, Xiao, Li, Siming, Sun, Yan, Ji, Chunli, Zhang, Chunhui, Jin-ai, Xue, Cui, Hongli, Li, Runzhi
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Frontiers Media SA
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022 |a 1664-462X 
024 7 |a 10.3389/fpls.2024.1395696  |2 doi 
035 |a 3273801432 
045 2 |b d20241001  |b d20241031 
100 1 |a Li, Ya-nan  |u College of Agriculture, Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Jinzhong, Shanxi, China 
245 1 |a Identification and expression analysis of calcium-dependent protein kinase family in oat (Avena sativa L.) and their functions in response to saline-alkali stresses 
260 |b Frontiers Media SA  |c Oct 2024 
513 |a Journal Article 
520 3 |a Calcium-dependent protein kinases (CDPKs) serve as calcium ion sensors and play crucial roles in all aspects of plant life cycle. While CDPK gene family has been extensively studied in various plants, there is limited information available for CDPK members in oat, an important cereal crop worldwide. Totally, 60 AsCDPK genes were identified in oat genome and were classified into four subfamilies based on their phylogenetic relationship. The members within each subfamily shared similar gene structure and conserved motifs. Collinearity analysis revealed that AsCDPK gene amplification was attributed to segmental duplication events and underwent strong purifying selection. AsCDPK promoters were predicted to contain cis -acting elements associated with hormones, biotic and abiotic stresses. AsCDPK gene expressions were induced by different salt stresses, exhibiting stress-specific under different salt treatments. Moreover, overexpression of AsCDPK26 gene enhanced salt resistance in C. reinhardtii , a single-cell photoautotrophic model plants. Further analysis revealed a significant correlation between AsCDPK26 and Na+/H+ antiporter 1 ( p <0.05), suggesting that AsCDPK26 may interact with ion transporter to modulate salt resistance. These results not only provide valuable insights into AsCDPK genes in response to different salt stresses, but also lay the foundation to mine novel candidates for improving salt tolerance in oat and other crops. 
653 |a Phylogenetics 
653 |a Gene amplification 
653 |a Protein kinase 
653 |a Flowers & plants 
653 |a Calcium 
653 |a Genes 
653 |a Amino acids 
653 |a Crops 
653 |a Kinases 
653 |a Cereal crops 
653 |a Stresses 
653 |a Proteins 
653 |a Salt tolerance 
653 |a Signal transduction 
653 |a Sensors 
653 |a Seeds 
653 |a Hormones 
653 |a Rice 
653 |a Abiotic stress 
653 |a Salt 
653 |a Phosphorylation 
653 |a Collinearity 
653 |a Plant growth 
653 |a Na+/H+-exchanging ATPase 
653 |a Salinity 
653 |a Salinity tolerance 
653 |a Calcium ions 
653 |a Environmental 
700 1 |a Lei, Chunyan  |u College of Agriculture, Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Jinzhong, Shanxi, China 
700 1 |a Yang, Qian  |u College of Agriculture, Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Jinzhong, Shanxi, China 
700 1 |a Yu, Xiao  |u College of Agriculture, Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Jinzhong, Shanxi, China 
700 1 |a Li, Siming  |u College of Agriculture, Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Jinzhong, Shanxi, China 
700 1 |a Sun, Yan  |u College of Agriculture, Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Jinzhong, Shanxi, China 
700 1 |a Ji, Chunli  |u College of Agriculture, Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Jinzhong, Shanxi, China 
700 1 |a Zhang, Chunhui  |u College of Agriculture, Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Jinzhong, Shanxi, China 
700 1 |a Jin-ai, Xue  |u College of Agriculture, Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Jinzhong, Shanxi, China 
700 1 |a Cui, Hongli  |u Key Laboratory of Coastal Biology and Bio-Resource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, Shandon, China 
700 1 |a Li, Runzhi  |u College of Agriculture, Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Jinzhong, Shanxi, China 
773 0 |t Frontiers in Plant Science  |g vol. 15 (Oct 2024), p. 1395696-1395718 
786 0 |d ProQuest  |t Agriculture Science Database 
856 4 1 |3 Citation/Abstract  |u https://www.proquest.com/docview/3273801432/abstract/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch 
856 4 0 |3 Full Text  |u https://www.proquest.com/docview/3273801432/fulltext/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch 
856 4 0 |3 Full Text - PDF  |u https://www.proquest.com/docview/3273801432/fulltextPDF/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch