In-Situ Self-Assembling Oligomeric Collagen Scaffold Enhances Vaccine Retention and Vaccine-Induced Humoral Immunity
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| 出版年: | Vaccines vol. 13, no. 11 (2025), p. 1146-1162 |
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| 第一著者: | |
| その他の著者: | , , , , |
| 出版事項: |
MDPI AG
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| 主題: | |
| オンライン･アクセス: | Citation/Abstract Full Text + Graphics Full Text - PDF |
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| 022 | |a 2076-393X | ||
| 024 | 7 | |a 10.3390/vaccines13111146 |2 doi | |
| 035 | |a 3275574072 | ||
| 045 | 2 | |b d20250101 |b d20251231 | |
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| 100 | 1 | |a Hernandez-Franco, Juan F |u Department of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USA; jfhernan@purdue.edu (J.F.H.-F.); casti134@purdue.edu (D.C.P.) | |
| 245 | 1 | |a In-Situ Self-Assembling Oligomeric Collagen Scaffold Enhances Vaccine Retention and Vaccine-Induced Humoral Immunity | |
| 260 | |b MDPI AG |c 2025 | ||
| 513 | |a Journal Article | ||
| 520 | 3 | |a Background/Objectives: Subunit vaccines composed of purified proteins and adjuvants offer excellent safety, but often generate short-lived immunity due to rapid antigen clearance and limited antigen-presenting cell engagement. Sustained, localized delivery of antigen and adjuvant may improve the magnitude and durability of the immune response without compromising safety. This study evaluated an in-situ polymerizing type I oligomeric collagen (Oligomer) scaffold to localize antigen/adjuvant at the injection site and prolong antigen presentation. Methods: Mice were immunized intramuscularly with ovalbumin (OVA) and CpG oligonucleotide adjuvant delivered alone or co-formulated with Oligomer. Antibody response and inflammation at the injection site were assessed post-booster at early (Day 32) and late (Day 68) time points. Antigen retention and dendritic cell trafficking to draining lymph nodes were evaluated using fluorescently labeled OVA. Results: The Oligomer scaffold retained vaccine antigen at the injection site without eliciting a material-mediated foreign body response. Co-delivery of OVA and CpG within the scaffold enhanced germinal center activity, increased follicular helper T cells and germinal center B cells, and skewed CD4+ T cells toward a Th1 phenotype. Humoral responses were greater and more durable, with higher OVA-specific IgG, IgG1, and IgG2a titers and an increased number of bone marrow antibody-secreting cells persisting through Day 68. Antigen-positive dendritic cells, including both resident and migratory subsets, were elevated in draining lymph nodes, indicating enhanced antigen transport. No anti-mouse collagen I antibodies were detected, confirming the maintenance of collagen self-tolerance. Conclusions: The Oligomer delivery platform functioned as a localized, immunotolerant vaccine depot, sustaining antigen availability and immune cell engagement. This spatiotemporal control enhanced germinal center responses and generated a more robust, durable humoral immune response, supporting its potential to improve subunit vaccine efficacy while maintaining an excellent safety profile. | |
| 651 | 4 | |a Indianapolis Indiana | |
| 651 | 4 | |a United States--US | |
| 653 | |a Collagen | ||
| 653 | |a Vaccines | ||
| 653 | |a Adjuvants | ||
| 653 | |a Immunity | ||
| 653 | |a Immune system | ||
| 653 | |a Lymphocytes T | ||
| 653 | |a Phenotypes | ||
| 653 | |a Aluminum | ||
| 653 | |a Antigen presentation | ||
| 653 | |a Antibody response | ||
| 653 | |a Antigens | ||
| 653 | |a Lymph nodes | ||
| 653 | |a Localization | ||
| 653 | |a Laboratory animals | ||
| 653 | |a Bone marrow | ||
| 653 | |a Lymphocytes | ||
| 653 | |a Oligomers | ||
| 653 | |a Immune clearance | ||
| 653 | |a Injection | ||
| 653 | |a Immune response | ||
| 653 | |a Lymphatic system | ||
| 653 | |a Immunoglobulin G | ||
| 653 | |a Collagen (type I) | ||
| 653 | |a Lymphocytes B | ||
| 653 | |a Enzymes | ||
| 653 | |a Ovalbumin | ||
| 653 | |a Biocompatibility | ||
| 653 | |a Antibodies | ||
| 653 | |a Germinal centers | ||
| 653 | |a Humoral immunity | ||
| 653 | |a Safety | ||
| 653 | |a CD4 antigen | ||
| 653 | |a Antigen-presenting cells | ||
| 653 | |a Oligonucleotides | ||
| 653 | |a Vaccine efficacy | ||
| 653 | |a Immunity (Disease) | ||
| 653 | |a Self-assembly | ||
| 653 | |a Immune response (humoral) | ||
| 653 | |a Durability | ||
| 653 | |a Immunological tolerance | ||
| 653 | |a Dendritic cells | ||
| 653 | |a Retention | ||
| 653 | |a Scaffolds | ||
| 653 | |a Immunization | ||
| 653 | |a Social | ||
| 700 | 1 | |a Gude Sushma |u Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA; sgude@purdue.edu (S.G.); morri107@purdue.edu (R.A.M.) | |
| 700 | 1 | |a Morrison, Rachel A |u Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA; sgude@purdue.edu (S.G.); morri107@purdue.edu (R.A.M.) | |
| 700 | 1 | |a Castillo Perez Daniela |u Department of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USA; jfhernan@purdue.edu (J.F.H.-F.); casti134@purdue.edu (D.C.P.) | |
| 700 | 1 | |a Voytik-Harbin, Sherry L |u Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA; sgude@purdue.edu (S.G.); morri107@purdue.edu (R.A.M.) | |
| 700 | 1 | |a HogenEsch Harm |u Department of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USA; jfhernan@purdue.edu (J.F.H.-F.); casti134@purdue.edu (D.C.P.) | |
| 773 | 0 | |t Vaccines |g vol. 13, no. 11 (2025), p. 1146-1162 | |
| 786 | 0 | |d ProQuest |t Biological Science Database | |
| 856 | 4 | 1 | |3 Citation/Abstract |u https://www.proquest.com/docview/3275574072/abstract/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text + Graphics |u https://www.proquest.com/docview/3275574072/fulltextwithgraphics/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text - PDF |u https://www.proquest.com/docview/3275574072/fulltextPDF/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |