The effects of ondansetron on diabetes and high-fat diet-induced liver disease: a critical role for protein tyrosine phosphatase 1B

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Publikašuvnnas:Frontiers in Pharmacology vol. 16 (Apr 2025), p. 1565628-1565644
Váldodahkki: Naeem, Fawad
Eará dahkkit: Aqeel, Maryam, Muhammad Ammar Zahid, Mustafeez Mujtaba Babar, Fawad Ali Shah, Agouni, Abdelali, Sohaib Zafar Malik
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Frontiers Media SA
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100 1 |a Naeem, Fawad  |u Riphah Institute of Pharmaceutical Sciences, Riphah International University, Islamabad, Pakistan, Shifa College of Pharmaceutical Sciences, Shifa Tameer-e-Millat University, Islamabad, Pakistan 
245 1 |a The effects of ondansetron on diabetes and high-fat diet-induced liver disease: a critical role for protein tyrosine phosphatase 1B 
260 |b Frontiers Media SA  |c Apr 2025 
513 |a Journal Article 
520 3 |a IntroductionThe escalating prevalence of diabetes and non-alcoholic fatty liver disease (NAFLD) has intensified the search for effective therapeutic interventions. The current study investigates the potential of ondansetron, a Food and Drug Administration (FDA)-approved drug for conditions like nausea and vomiting, as a novel treatment option for these metabolic disorders.MethodsA multifaceted approach, encompassing computational analyses, in vitro enzyme inhibition assays, and in vivo experiments in a high-fat diet (HFD)-induced disease model in rats were employed.ResultsComputational studies, including pharmacophore modeling, molecular docking, and molecular dynamics (MD) simulations, revealed the strong binding affinity of ondansetron to the allosteric site of protein tyrosine phosphatase 1B (PTP1B), a key regulator of insulin and lipid homeostasis. The in vitro enzyme inhibition assay further confirmed ondansetron’s ability to directly inhibit PTP1B activity. Animal experiments demonstrated ondansetron’s antihyperglycemic effects, reducing blood glucose levels and improving insulin sensitivity in HFD-fed rats. The drug also exhibited hepatoprotective properties, mitigating liver damage and improving tissue architecture. Additionally, ondansetron’s anti-inflammatory and antioxidant activities were evident in its ability to reduce pro-inflammatory markers and oxidative stress in the liver.DiscussionThese therapeutic effects position ondansetron as a promising candidate for further investigation in clinical settings for the treatment of diabetes and NAFLD and, hence, support the use of the drug repurposing approach for addressing the growing burden of metabolic diseases. 
610 4 |a Food & Drug Administration--FDA 
651 4 |a United Kingdom--UK 
651 4 |a New York 
651 4 |a United States--US 
653 |a Diabetes 
653 |a Insulin resistance 
653 |a Hydrogen bonds 
653 |a High fat diet 
653 |a Binding sites 
653 |a Fatty liver 
653 |a Drug development 
653 |a Blood levels 
653 |a Computer applications 
653 |a Metabolism 
653 |a Inflammation 
653 |a Liver diseases 
653 |a Apoptosis 
653 |a Metabolic disorders 
653 |a Proteins 
653 |a Protein-tyrosine-phosphatase 
653 |a Therapeutic applications 
653 |a Simulation 
653 |a Phosphatase 
653 |a Insulin 
653 |a FDA approval 
653 |a Oxidative stress 
653 |a Glucose 
653 |a Diabetes mellitus 
653 |a Tumor necrosis factor-TNF 
653 |a Ligands 
653 |a Homeostasis 
653 |a Allosteric properties 
653 |a Enzymes 
700 1 |a Aqeel, Maryam  |u Riphah Institute of Pharmaceutical Sciences, Riphah International University, Islamabad, Pakistan 
700 1 |a Muhammad Ammar Zahid  |u Department of Pharmaceutical Sciences, College of Pharmacy, QU Health, Qatar University, Doha, Qatar 
700 1 |a Mustafeez Mujtaba Babar  |u Shifa College of Pharmaceutical Sciences, Shifa Tameer-e-Millat University, Islamabad, Pakistan 
700 1 |a Fawad Ali Shah  |u Department of Pharmacology and Toxicology, College of Pharmacy, Prince Sattam bin Abdulaziz University, Al-Kharj, Saudi Arabia 
700 1 |a Agouni, Abdelali  |u Department of Pharmaceutical Sciences, College of Pharmacy, QU Health, Qatar University, Doha, Qatar 
700 1 |a Sohaib Zafar Malik  |u Riphah Institute of Pharmaceutical Sciences, Riphah International University, Islamabad, Pakistan 
773 0 |t Frontiers in Pharmacology  |g vol. 16 (Apr 2025), p. 1565628-1565644 
786 0 |d ProQuest  |t Biological Science Database 
856 4 1 |3 Citation/Abstract  |u https://www.proquest.com/docview/3279124235/abstract/embedded/L8HZQI7Z43R0LA5T?source=fedsrch 
856 4 0 |3 Full Text  |u https://www.proquest.com/docview/3279124235/fulltext/embedded/L8HZQI7Z43R0LA5T?source=fedsrch 
856 4 0 |3 Full Text - PDF  |u https://www.proquest.com/docview/3279124235/fulltextPDF/embedded/L8HZQI7Z43R0LA5T?source=fedsrch