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Quercetin in Shengxian Decoction exhibits anti-ferroptosis protective roles in a myocardial infarction model via targeting DPP4/ HMOX1, based on network pharmacology and molecular docking

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Publicat a:Frontiers in Pharmacology vol. 16 (Apr 2025), p. 1583509-1583526
Autor principal: Zhai, Yuming
Altres autors: Fu, Jiamei, Yang, Jianfei, Zhou, Yabin
Publicat:
Frontiers Media SA
Matèries:
Beijing China
China
Databases
Ferroptosis
Cell death
Morbidity
Disease
Mortality
Heart failure
Hypoxia
Traditional Chinese medicine
Drug development
Computer simulation
Pharmacology
Cobalt chloride
Myocardial infarction
Genes
Apoptosis
Cell culture
Drug dosages
Proteins
Heart attacks
Permeability
Cobalt
Cardiomyocytes
Pharmacokinetics
Heme oxygenase (decyclizing)
Gene expression
Quercetin
Cell viability
Ischemia
Cardiac function
Accés en línia:Citation/Abstract
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Resum:BackgroundMyocardial infarction (MI) is characterized by high morbidity. In this study, we aimed to elucidate potential targets of Shengxian Decoction (SXD) against MI.MethodsPairing of SXD active ingredients and MI targets was conducted using the Chinese Medicine System Pharmacological Database, Gene Expression Omnibus (GEO), and STRING databases. The effects of SXD on MI were validated in vitro . Molecular docking was verified using cellular thermal shift assay (CETSA).ResultsA total of 40 active ingredients and 28 MI-related targets were obtained. Cross-analysis on 28 targets and cell death-related genes identified two crucial ferroptosis-related targets, namely, dipeptidyl peptidase 4 (DPP4) and heme oxygenase 1 (HMOX1). In cobalt chloride (CoCl2)-induced hypoxic H9c2 cells, SXD could remarkably improve cell viability and inhibit cell death. Meanwhile, SXD treatment significantly affected the ferroptosis-related markers in hypoxic H9c2 cells. Molecular docking and CETSA results showed that quercetin had good binding activity with DPP4 and HMOX1.ConclusionImportant active ingredient quercetin in SXD could exert anti-ferroptosis protective roles on MI through targeting ferroptosis-related genes (DPP4/HMOX1), thereby contributing to the protective role of SXD on MI.
ISSN:1663-9812
DOI:1583509
Font:Biological Science Database