Detection and risk factor analysis of avian colibacillosis associated with colistin-resistant Escherichia coli and Klebsiella pneumoniae
Guardado en:
| Udgivet i: | Frontiers in Veterinary Science vol. 12 (Jul 2025), p. 1612542-1612554 |
|---|---|
| Hovedforfatter: | |
| Andre forfattere: | , , , , , , , , , |
| Udgivet: |
Frontiers Media SA
|
| Fag: | |
| Online adgang: | Citation/Abstract Full Text Full Text - PDF |
| Tags: |
Ingen Tags, Vær først til at tagge denne postø!
|
MARC
| LEADER | 00000nab a2200000uu 4500 | ||
|---|---|---|---|
| 001 | 3280620472 | ||
| 003 | UK-CbPIL | ||
| 022 | |a 2297-1769 | ||
| 024 | 7 | |a 10.3389/fvets.2025.1612542 |2 doi | |
| 035 | |a 3280620472 | ||
| 045 | 2 | |b d20250701 |b d20250731 | |
| 100 | 1 | |a Saeed, Muhammad Adnan |u Department of Pathobiology (Microbiology Section), University of Veterinary and Animal Sciences, Lahore, Pakistan | |
| 245 | 1 | |a Detection and risk factor analysis of avian colibacillosis associated with colistin-resistant Escherichia coli and Klebsiella pneumoniae | |
| 260 | |b Frontiers Media SA |c Jul 2025 | ||
| 513 | |a Journal Article | ||
| 520 | 3 | |a Colibacillosis associated with colistin-resistant avian pathogenic Escherichia coli ( E. coli ) poses a threat to both food security and public health. The potential horizontal transmission of mobilized colistin-resistant ( mcr ) genes facilitates the co-emergence of Klebsiella pneumoniae . This study aimed to determine the prevalence, molecular detection, analyze the antibiogram and identify associated risk factors for colistin-resistant E. coli and Klebsiella pneumoniae isolated from broiler chicken in three districts of Punjab province, Pakistan. In total, 230 visceral organ samples were collected from 13 different chicken farms located in Sargodha, Jhang and Toba Tek Singh in Pakistan. Following isolation, the broth microdilution test was used to confirm phenotypic colistin resistance. Polymerase chain reaction was used to detect mcr- 1 and mcr- 2 genes associated with colistin resistance. Antimicrobial susceptibility test against 11 antibiotics was performed using the Kirby-Bauer disk diffusion method. Risk factors associated with colistin-resistant bacteria, including host attributes, farm management practices, environmental and agent characteristics, were analyzed. The prevalence of colistin-resistant E. coli and K. pneumoniae was 24.78% (95% CI, 19.6–30.7%) and 3.04% (95% CI, 1.5–6.1%), respectively. The prevalence of colistin-resistant E. coli varied between cities at 42, 23.61 and 5.55% for Jhang, Sargodha and Toba Tek Singh, respectively. The detection frequency of mcr- 1 gene, 42.1% (24/57), was significantly ( p < 0.01) higher than that of the mcr- 2 gene, 14.03% (8/57). Phylogenetic analysis of lipid A phosphoethanolamine transferase sequences revealed greater similarity with mcr- 1.5 variant. Isolates were found resistant to amoxicillin-clavulanic acid (84.21%), cefotaxime (70.17%), and trimethoprim-sulfamethoxazole (73.68%). The multivariate logistic regression predicted preceding viral infection of the respiratory tract as a significant association (OR = 4.808, p < 0.01), whereas daily removal/culling of dead/diseased chicken (OR = 0.308, p = 0.01) was a protective factor against the emergence of colistin-resistant strains. These findings indicate that the emergence of colistin-resistant strains deteriorate colibacillosis control efforts in poultry and serves as a possible reservoir for zoonotic infections. | |
| 651 | 4 | |a United Kingdom--UK | |
| 651 | 4 | |a United States--US | |
| 651 | 4 | |a Lahore Pakistan | |
| 651 | 4 | |a Pakistan | |
| 653 | |a Vaccines | ||
| 653 | |a Klebsiella | ||
| 653 | |a Agricultural practices | ||
| 653 | |a Clavulanic acid | ||
| 653 | |a Biosecurity | ||
| 653 | |a Antibiotics | ||
| 653 | |a Immunization | ||
| 653 | |a Farm management | ||
| 653 | |a Food security | ||
| 653 | |a Environmental management | ||
| 653 | |a Acid resistance | ||
| 653 | |a Trimethoprim | ||
| 653 | |a Risk factors | ||
| 653 | |a Chickens | ||
| 653 | |a Trimethoprim-sulfamethoxazole | ||
| 653 | |a Lipid A | ||
| 653 | |a E coli | ||
| 653 | |a Public health | ||
| 653 | |a Strains (organisms) | ||
| 653 | |a Animal sciences | ||
| 653 | |a Pericarditis | ||
| 653 | |a Peritonitis | ||
| 653 | |a Food contamination & poisoning | ||
| 653 | |a Genes | ||
| 653 | |a Bacteria | ||
| 653 | |a Colibacillosis | ||
| 653 | |a Factor analysis | ||
| 653 | |a Disease prevention | ||
| 653 | |a Gram-negative bacteria | ||
| 653 | |a Cefotaxime | ||
| 653 | |a Drug resistance | ||
| 653 | |a Horizontal transmission (disease) | ||
| 653 | |a Colistin | ||
| 653 | |a Sulfamethoxazole | ||
| 653 | |a Polymerase chain reaction | ||
| 653 | |a Farms | ||
| 653 | |a Bacterial infections | ||
| 653 | |a Respiratory tract | ||
| 653 | |a Antimicrobial agents | ||
| 653 | |a Lipids | ||
| 653 | |a Poultry farming | ||
| 653 | |a Poultry | ||
| 653 | |a Escherichia coli | ||
| 653 | |a Klebsiella pneumoniae | ||
| 700 | 1 | |a Haseeb Asif |u Department of Pathobiology (Microbiology Section), University of Veterinary and Animal Sciences, Lahore, Pakistan | |
| 700 | 1 | |a Ehtisham-ul-Haque, Syed |u Department of Pathobiology (Microbiology Section), University of Veterinary and Animal Sciences, Lahore, Pakistan | |
| 700 | 1 | |a Khan, Aman Ullah |u Department of Pathobiology (Microbiology Section), University of Veterinary and Animal Sciences, Lahore, Pakistan | |
| 700 | 1 | |a Aziz ur Rehman |u Department of Pathobiology (Pathology Section), University of Veterinary and Animal Sciences, Lahore, Pakistan | |
| 700 | 1 | |a Rehman, Aiman |u Department of Pathobiology (Microbiology Section), University of Veterinary and Animal Sciences, Lahore, Pakistan | |
| 700 | 1 | |a Muhammad Kamran Rafique |u Department of Pathobiology (Pathology Section), University of Veterinary and Animal Sciences, Lahore, Pakistan | |
| 700 | 1 | |a Ahmed, Ishtiaq |u Department of Pathobiology (Pathology Section), University of Veterinary and Animal Sciences, Lahore, Pakistan | |
| 700 | 1 | |a Qamar, Muhammad Fiaz |u Department of Pathobiology (Parasitology Section), University of Veterinary and Animal Sciences, Lahore, Pakistan | |
| 700 | 1 | |a Herbert, Tomaso |u Institute of Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institut, Jena, Germany | |
| 700 | 1 | |a El-Adawy, Hosny |u Institute of Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institut, Jena, Germany | |
| 773 | 0 | |t Frontiers in Veterinary Science |g vol. 12 (Jul 2025), p. 1612542-1612554 | |
| 786 | 0 | |d ProQuest |t Agriculture Science Database | |
| 856 | 4 | 1 | |3 Citation/Abstract |u https://www.proquest.com/docview/3280620472/abstract/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text |u https://www.proquest.com/docview/3280620472/fulltext/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text - PDF |u https://www.proquest.com/docview/3280620472/fulltextPDF/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |