An Antioxidative Exopolysaccharide–Protein Complex of Cordyceps Cs-HK1 Fungus and Its Epithelial Barrier-Protective Effects in Caco-2 Cell Culture
Guardat en:
| Publicat a: | Antioxidants vol. 14, no. 12 (2025), p. 1501-1525 |
|---|---|
| Autor principal: | |
| Altres autors: | , , , , , |
| Publicat: |
MDPI AG
|
| Matèries: | |
| Accés en línia: | Citation/Abstract Full Text + Graphics Full Text - PDF |
| Etiquetes: |
Sense etiquetes, Sigues el primer a etiquetar aquest registre!
|
MARC
| LEADER | 00000nab a2200000uu 4500 | ||
|---|---|---|---|
| 001 | 3286254753 | ||
| 003 | UK-CbPIL | ||
| 022 | |a 2076-3921 | ||
| 024 | 7 | |a 10.3390/antiox14121501 |2 doi | |
| 035 | |a 3286254753 | ||
| 045 | 2 | |b d20250101 |b d20251231 | |
| 084 | |a 231337 |2 nlm | ||
| 100 | 1 | |a Zhu Yan Yu | |
| 245 | 1 | |a An Antioxidative Exopolysaccharide–Protein Complex of <i>Cordyceps</i> Cs-HK1 Fungus and Its Epithelial Barrier-Protective Effects in Caco-2 Cell Culture | |
| 260 | |b MDPI AG |c 2025 | ||
| 513 | |a Journal Article | ||
| 520 | 3 | |a The exopolysaccharides (EPS) from the mycelial fermentation of Cordyceps sinensis Cs-HK1, especially the low-molecular weight, protein-rich exopolysaccharide fractions (EPS-LM), have previously exhibited significant antioxidant activity. This study further investigated the antioxidant and protective effects of EPS-LM on intestinal epithelial barrier integrity in Caco-2 monolayers challenged with hydrogen peroxide (H2O2, 550 μM). EPS-LM contained two major molecular-weight fractions, 25 kDa and 1.7 kDa, with 19.3% total carbohydrate and 28.7% protein content (w/w). Treatment of the cells with EPS-LM (50–200 μg/mL) showed concentration-dependent protective effects against ROS-induced losses of cell viability and epithelial barrier integrity. EPS-LM treatment enhanced the activities of major antioxidant enzymes (SOD, GSH-Px, and CAT) and modulated NRF2 and its downstream target NQO1, consistent with alleviated oxidative stress. It also improved several indicators of intestinal barrier function, including increased transepithelial electrical resistance and upregulation of tight junction proteins (Occludin, ZO-1, and Claudin-1). These results provide new experimental evidence and theoretical basis for the nutraceutical potential of EPS-LM to mitigate oxidative stress and preserve intestinal epithelial barrier integrity. | |
| 610 | 4 | |a Agilent Technologies Inc | |
| 651 | 4 | |a United States--US | |
| 653 | |a Fungi | ||
| 653 | |a Functional foods & nutraceuticals | ||
| 653 | |a Cell culture | ||
| 653 | |a Carbohydrates | ||
| 653 | |a Amino acids | ||
| 653 | |a Antioxidants | ||
| 653 | |a Inflammation | ||
| 653 | |a Chromatography | ||
| 653 | |a Electrical resistivity | ||
| 653 | |a Zonula occludens-1 protein | ||
| 653 | |a Ethanol | ||
| 653 | |a Oxidative stress | ||
| 653 | |a Proteins | ||
| 653 | |a Intestine | ||
| 653 | |a Fermentation | ||
| 653 | |a Exopolysaccharides | ||
| 653 | |a Permeability | ||
| 653 | |a Molecular weight | ||
| 653 | |a Cell viability | ||
| 653 | |a Polyethylene glycol | ||
| 653 | |a Irritable bowel syndrome | ||
| 653 | |a Hydrogen peroxide | ||
| 700 | 1 | |a Wu Margaret M. H. | |
| 700 | 1 | |a Zhao Zi Chen | |
| 700 | 1 | |a Gu, Fang Ting | |
| 700 | 1 | |a Huang Lin Xi | |
| 700 | 1 | |a Kwok Kevin W. H. | |
| 700 | 1 | |a Wu, Jian Yong | |
| 773 | 0 | |t Antioxidants |g vol. 14, no. 12 (2025), p. 1501-1525 | |
| 786 | 0 | |d ProQuest |t Biological Science Database | |
| 856 | 4 | 1 | |3 Citation/Abstract |u https://www.proquest.com/docview/3286254753/abstract/embedded/J7RWLIQ9I3C9JK51?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text + Graphics |u https://www.proquest.com/docview/3286254753/fulltextwithgraphics/embedded/J7RWLIQ9I3C9JK51?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text - PDF |u https://www.proquest.com/docview/3286254753/fulltextPDF/embedded/J7RWLIQ9I3C9JK51?source=fedsrch |