Cytocompatibility and Microbiological Effects of Ti6Al4V Particles Generated During Implantoplasty on Human Fibroblasts, Osteoblasts, and Multispecies Oral Biofilm
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| Udgivet i: | Materials vol. 18, no. 24 (2025), p. 5626-5644 |
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| Hovedforfatter: | |
| Andre forfattere: | , , , , , , , |
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MDPI AG
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| 001 | 3286317871 | ||
| 003 | UK-CbPIL | ||
| 022 | |a 1996-1944 | ||
| 024 | 7 | |a 10.3390/ma18245626 |2 doi | |
| 035 | |a 3286317871 | ||
| 045 | 2 | |b d20250101 |b d20251231 | |
| 084 | |a 231532 |2 nlm | ||
| 100 | 1 | |a Vegas-Bustamante, Erika |u School of Medicine and Health Sciences, Campus de Bellvitge, Universitat de Barcelona, C/Feixa Llarga s/n, Pavelló Govern 2ª Planta, Despatx 2.9, 08907 L’Hospitalet de Llobregat, Spain; erikavegasbustamante@ub.edu (E.V.-B.); masanchezg@ub.edu (M.Á.S.-G.); gemmasanmarti@ub.edu (G.S.-G.); ocamps@ub.edu (O.C.-F.) | |
| 245 | 1 | |a Cytocompatibility and Microbiological Effects of Ti6Al4V Particles Generated During Implantoplasty on Human Fibroblasts, Osteoblasts, and Multispecies Oral Biofilm | |
| 260 | |b MDPI AG |c 2025 | ||
| 513 | |a Journal Article | ||
| 520 | 3 | |a Objectives: This study aimed to evaluate the cytotoxic effects of Ti6Al4V particles and implantoplasty (IP)-treated surfaces on human fibroblasts and osteoblasts, and to investigate the influence of these particles on multispecies oral biofilm formation. Methods: Ti6Al4V particles generated during implantoplasty were collected. Human fibroblasts (HFF-1) and osteoblast-like cells (SaOs-2) were used to assess cytotoxicity through indirect lactate dehydrogenase (LDH) assays. Multispecies biofilms composed of Streptococcus oralis, Actinomyces viscosus, Veillonella parvula and Porphyromonas gingivalis were evaluated based on colony-forming units (CFUs) and metabolic activity. Fibroblasts and osteoblasts were co-cultured with biofilm-contaminated particles for 2, 4 and 6 h. Cell morphology and biofilm association were examined by phase-contrast microscopy, while metabolic activity was measured spectrophotometrically. Results: IP-treated surfaces showed no significant cytotoxicity (metabolic activity > 92%, LDH < 20%). Ti6Al4V particles selectively promoted A. viscosus and V. parvula growth (metabolic activity increases of ≈192% and ≈203%; CFU significantly higher versus controls, p < 0.05). Co-culture with biofilm-contaminated particles drastically reduced cell activity (fibroblasts < 25%, osteoblasts < 10%), whereas bacteria-free particles did not. Conclusions: Biofilm-contaminated particles released during implantoplasty markedly impair fibroblast and osteoblast cytocompatibility and selectively alter bacterial growth, whereas IP-treated surfaces per se are biocompatible. Minimizing particle dissemination and bacterial contamination during IP is therefore crucial. | |
| 651 | 4 | |a Spain | |
| 651 | 4 | |a United States--US | |
| 651 | 4 | |a Germany | |
| 653 | |a Titanium base alloys | ||
| 653 | |a Homeostasis | ||
| 653 | |a Dental implants | ||
| 653 | |a Silicones | ||
| 653 | |a Toxicity | ||
| 653 | |a Fibroblasts | ||
| 653 | |a Osteoblasts | ||
| 653 | |a Biofilms | ||
| 653 | |a Lactate dehydrogenase | ||
| 653 | |a Spectrophotometry | ||
| 653 | |a Cytotoxicity | ||
| 653 | |a Titanium | ||
| 653 | |a Cytokines | ||
| 653 | |a Tumor necrosis factor-TNF | ||
| 653 | |a Biocompatibility | ||
| 653 | |a Metabolism | ||
| 653 | |a Metabolites | ||
| 653 | |a Evaluation | ||
| 653 | |a Bacteria | ||
| 700 | 1 | |a Toledano-Serrabona Jorge |u School of Medicine and Health Sciences, Campus de Bellvitge, Universitat de Barcelona, C/Feixa Llarga s/n, Pavelló Govern 2ª Planta, Despatx 2.9, 08907 L’Hospitalet de Llobregat, Spain; erikavegasbustamante@ub.edu (E.V.-B.); masanchezg@ub.edu (M.Á.S.-G.); gemmasanmarti@ub.edu (G.S.-G.); ocamps@ub.edu (O.C.-F.) | |
| 700 | 1 | |a Sánchez-Garcés, María Ángeles |u School of Medicine and Health Sciences, Campus de Bellvitge, Universitat de Barcelona, C/Feixa Llarga s/n, Pavelló Govern 2ª Planta, Despatx 2.9, 08907 L’Hospitalet de Llobregat, Spain; erikavegasbustamante@ub.edu (E.V.-B.); masanchezg@ub.edu (M.Á.S.-G.); gemmasanmarti@ub.edu (G.S.-G.); ocamps@ub.edu (O.C.-F.) | |
| 700 | 1 | |a Figueiredo Rui |u School of Medicine and Health Sciences, Campus de Bellvitge, Universitat de Barcelona, C/Feixa Llarga s/n, Pavelló Govern 2ª Planta, Despatx 2.9, 08907 L’Hospitalet de Llobregat, Spain; erikavegasbustamante@ub.edu (E.V.-B.); masanchezg@ub.edu (M.Á.S.-G.); gemmasanmarti@ub.edu (G.S.-G.); ocamps@ub.edu (O.C.-F.) | |
| 700 | 1 | |a Demiquels-Punzano Elena |u Bioengineering Institute of Technology (BIT), Universitat International de Catalunya, 08017 Barcelona, Spain; elenademiquels@uic.es | |
| 700 | 1 | |a Gil, Javier |u Bionspired Oral Biomaterials Interfaces, Departament de Ciencia e Ingenieria de Materiales, Escola d’Engingeria Barcelona Est, Universitat Politécnica de Catalunya, Av. Eduard Maristany 16, 08019 Barcelona, Spain | |
| 700 | 1 | |a Delgado, Luis M |u Bionspired Oral Biomaterials Interfaces, Departament de Ciencia e Ingenieria de Materiales, Escola d’Engingeria Barcelona Est, Universitat Politécnica de Catalunya, Av. Eduard Maristany 16, 08019 Barcelona, Spain | |
| 700 | 1 | |a Sanmartí-García Gemma |u School of Medicine and Health Sciences, Campus de Bellvitge, Universitat de Barcelona, C/Feixa Llarga s/n, Pavelló Govern 2ª Planta, Despatx 2.9, 08907 L’Hospitalet de Llobregat, Spain; erikavegasbustamante@ub.edu (E.V.-B.); masanchezg@ub.edu (M.Á.S.-G.); gemmasanmarti@ub.edu (G.S.-G.); ocamps@ub.edu (O.C.-F.) | |
| 700 | 1 | |a Camps-Font Octavi |u School of Medicine and Health Sciences, Campus de Bellvitge, Universitat de Barcelona, C/Feixa Llarga s/n, Pavelló Govern 2ª Planta, Despatx 2.9, 08907 L’Hospitalet de Llobregat, Spain; erikavegasbustamante@ub.edu (E.V.-B.); masanchezg@ub.edu (M.Á.S.-G.); gemmasanmarti@ub.edu (G.S.-G.); ocamps@ub.edu (O.C.-F.) | |
| 773 | 0 | |t Materials |g vol. 18, no. 24 (2025), p. 5626-5644 | |
| 786 | 0 | |d ProQuest |t Materials Science Database | |
| 856 | 4 | 1 | |3 Citation/Abstract |u https://www.proquest.com/docview/3286317871/abstract/embedded/6A8EOT78XXH2IG52?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text + Graphics |u https://www.proquest.com/docview/3286317871/fulltextwithgraphics/embedded/6A8EOT78XXH2IG52?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text - PDF |u https://www.proquest.com/docview/3286317871/fulltextPDF/embedded/6A8EOT78XXH2IG52?source=fedsrch |