Unravelling the Genomic and Virulence Diversity of Legionella pneumophila Strains Isolated from Anthropogenic Water Systems
I tiakina i:
| I whakaputaina i: | Microorganisms vol. 13, no. 12 (2025), p. 2832-2853 |
|---|---|
| Kaituhi matua: | |
| Ētahi atu kaituhi: | , , , , |
| I whakaputaina: |
MDPI AG
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| Ngā marau: | |
| Urunga tuihono: | Citation/Abstract Full Text + Graphics Full Text - PDF |
| Ngā Tūtohu: |
Kāore He Tūtohu, Me noho koe te mea tuatahi ki te tūtohu i tēnei pūkete!
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MARC
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| 001 | 3286330536 | ||
| 003 | UK-CbPIL | ||
| 022 | |a 2076-2607 | ||
| 024 | 7 | |a 10.3390/microorganisms13122832 |2 doi | |
| 035 | |a 3286330536 | ||
| 045 | 2 | |b d20250101 |b d20251231 | |
| 084 | |a 231538 |2 nlm | ||
| 100 | 1 | |a Barigelli Sofia |u Laboratory of Applied and Environmental Microbiology, Department of Chemistry, Biology and Biotechnology, University of Perugia, 06122 Perugia, Italy; sofia.barigelli@dottorandi.unipg.it (S.B.); maya.petricciuolo@unipg.it (M.P.) | |
| 245 | 1 | |a Unravelling the Genomic and Virulence Diversity of <i>Legionella pneumophila</i> Strains Isolated from Anthropogenic Water Systems | |
| 260 | |b MDPI AG |c 2025 | ||
| 513 | |a Journal Article | ||
| 520 | 3 | |a Legionella pneumophila, a waterborne pathogen naturally present in freshwater and capable of colonizing artificial water systems, is responsible for Legionnaires’ disease (LD), a severe form of pneumonia transmitted through inhalation of contaminated aerosols. Virulence of Legionella strains is affected by the plasticity of their genome, shaped by horizontal gene transfer and recombination events. Thus, contaminated water systems can host diverse Legionella populations with a distinct virulence potential. Here, we compare the genomic diversity of Legionella pneumophila strains isolated in water systems of academic buildings, together with their cytotoxicity and intracellular replication in THP-1-like macrophages. A six-year environmental surveillance revealed Legionella pneumophila contamination in 20 out of the 50 monitored sites, identifying five serogroups (sg) and 13 Sequence Types (STs). Phylogenetic investigations based on core genome multilocus sequence typing (cgMLST) and comparative genomics of representative isolates of each ST showed a broad diversity and a heterogeneous virulence repertoire, especially within the Dot/Icm and Lvh secretion systems. Following macrophage infection, a strain-dependent cytotoxicity and intracellular replication was observed, underlying significant pathogenic diversity within the same species and stage-dependent infection dynamics. Together, these results showed strain-specific genetic and phenotypic virulence traits to be considered during risk assessment in environmental surveillance. | |
| 651 | 4 | |a Italy | |
| 651 | 4 | |a United States--US | |
| 653 | |a Infections | ||
| 653 | |a Cytotoxicity | ||
| 653 | |a Pathogens | ||
| 653 | |a Replication | ||
| 653 | |a Bacterial diseases | ||
| 653 | |a Surveillance | ||
| 653 | |a Inhalation | ||
| 653 | |a Genomics | ||
| 653 | |a Epidemiology | ||
| 653 | |a Water pollution | ||
| 653 | |a Aerosols | ||
| 653 | |a Species diversity | ||
| 653 | |a Legionnaire's disease | ||
| 653 | |a Drinking water | ||
| 653 | |a Risk assessment | ||
| 653 | |a Nucleotide sequence | ||
| 653 | |a Genetic diversity | ||
| 653 | |a Genes | ||
| 653 | |a Toxicity | ||
| 653 | |a Macrophages | ||
| 653 | |a Virulence | ||
| 653 | |a Horizontal transfer | ||
| 653 | |a Disinfection & disinfectants | ||
| 653 | |a Respiration | ||
| 653 | |a Cooling | ||
| 653 | |a Public buildings | ||
| 653 | |a Gene transfer | ||
| 653 | |a Genetic engineering | ||
| 653 | |a Legionnaires' disease bacterium | ||
| 653 | |a Whole genome sequencing | ||
| 653 | |a Public health | ||
| 653 | |a Multilocus sequence typing | ||
| 653 | |a Intracellular | ||
| 653 | |a Legionella | ||
| 653 | |a Legionella pneumophila | ||
| 700 | 1 | |a Koper Piotr |u Department of Genetics and Microbiology, Institute of Biological Sciences, Maria Curie-Skłodowska University, 20-033 Lublin, Poland; piotr.koper@mail.umcs.pl (P.K.); marta.palusinska-szysz@mail.umcs.pl (M.P.-S.) | |
| 700 | 1 | |a Petricciuolo Maya |u Laboratory of Applied and Environmental Microbiology, Department of Chemistry, Biology and Biotechnology, University of Perugia, 06122 Perugia, Italy; sofia.barigelli@dottorandi.unipg.it (S.B.); maya.petricciuolo@unipg.it (M.P.) | |
| 700 | 1 | |a Firrincieli Andrea |u Department for Innovation in Biological, Agro-Food and Forest Systems, University of Tuscia, 01100 Viterbo, Italy; andrea.firrincieli@unitus.it | |
| 700 | 1 | |a Palusińska-Szysz Marta |u Department of Genetics and Microbiology, Institute of Biological Sciences, Maria Curie-Skłodowska University, 20-033 Lublin, Poland; piotr.koper@mail.umcs.pl (P.K.); marta.palusinska-szysz@mail.umcs.pl (M.P.-S.) | |
| 700 | 1 | |a Federici Ermanno |u Laboratory of Applied and Environmental Microbiology, Department of Chemistry, Biology and Biotechnology, University of Perugia, 06122 Perugia, Italy; sofia.barigelli@dottorandi.unipg.it (S.B.); maya.petricciuolo@unipg.it (M.P.) | |
| 773 | 0 | |t Microorganisms |g vol. 13, no. 12 (2025), p. 2832-2853 | |
| 786 | 0 | |d ProQuest |t Biological Science Database | |
| 856 | 4 | 1 | |3 Citation/Abstract |u https://www.proquest.com/docview/3286330536/abstract/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text + Graphics |u https://www.proquest.com/docview/3286330536/fulltextwithgraphics/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text - PDF |u https://www.proquest.com/docview/3286330536/fulltextPDF/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |