Change in functional connectivity strength during rest and encoding is differentially related to Alzheimer's pathology and memory depending on APOE genotype

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Udgivet i:Alzheimer's & Dementia vol. 21 (Dec 1, 2025)
Hovedforfatter: Fischer, Larissa
Andre forfattere: Adams, Jenna N., Molloy, Eóin N., Tremblay‐Mercier, Jennifer, Remz, Jordana, Binette, Alexa Pichet, Rajah, Natasha, Villeneuve, Sylvia, Maass, Anne
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022 |a 1552-5260 
022 |a 1552-5279 
024 7 |a 10.1002/alz70856_100192  |2 doi 
035 |a 3286636149 
045 0 |b d20251201 
100 1 |a Fischer, Larissa  |u German Center for Neurodegenerative Diseases (DZNE), Magdeburg, Germany, 
245 1 |a Change in functional connectivity strength during rest and encoding is differentially related to Alzheimer's pathology and memory depending on APOE genotype 
260 |b John Wiley & Sons, Inc.  |c Dec 1, 2025 
513 |a Journal Article 
520 3 |a Background The medial temporal lobe (MTL) and posteromedial cortex (PMC) are essential for episodic memory and affected early by Alzheimer's pathology, particularly in APOE4 carriers. Functional connectivity (FC) changes within and between MTL and PMC could be detrimental or beneficial for cognition. However, the relation of those changes to Alzheimer's pathology and memory performance is unclear and most studies assess FC only during rest. We hypothesized that increasing FC strength would be associated with higher pathology burden, especially in APOE4 carriers. Method In this preregistered study, we analysed longitudinal 3‐Tesla fMRI over up to 4 years and cross‐sectional amyloid‐beta and tau PET (PREVENT‐AD cohort; details in Figure 1). We assessed changes in resting‐state FC (RSFC) and task‐FC during intentional object‐location encoding within (ΔFCPMC, ΔFCMTL) and between MTL and PMC (ΔFCMTL‐PMC). The sample included 152 cognitively unimpaired older adults (63±5years, 102 female, 59 APOE4). We investigated associations between ΔFC strength with i) pathology burden and ii) change in delayed memory (RBANS composite score and fMRI‐task object recognition), and interactions with APOE genotype. We used multiple regression and linear mixed models including APOE, age, sex and education. Result We found ΔFC by APOE interactions predicting pathology. Specifically, declining RSFCPMC (p = 0.038; Figure 2a) was related to more global amyloid in APOE4 carriers only. In contrast, increasing encoding‐FCMTL was related to more entorhinal tau in APOE4 carriers only (p = 0.032, Figure 2b). Regarding cognition, regardless of APOE status, increasing encoding‐FCPMC was related to decreasing RBANS (p = 0.018) performance and object recognition (p = 0.001). Finally, increasing RSFCMTL‐PMC was related to increasing RBANS performance (p = 0.032; Figure 3a), but increasing encoding‐FCMTL‐PMC was related to decreasing object recognition (p = 0.014; Figure 3b). Conclusion Our study shows APOE‐dependent and region‐specific associations of ΔFC strength within and between episodic memory areas with pathology burden and memory performance. Notably, associations differed between RSFC and task‐FC. In APOE4 carriers, longitudinally increasing FC or “hyperconnectivity” within MTL during encoding was related to tau in line with our hypothesis. However, in PMC, longitudinally decreasing FC during rest was related to more amyloid, indicating a disconnection in PMC regions. Our study highlights that pathology‐related network changes manifest differentially during rest and task (memory encoding). 
653 |a Episodic memory 
653 |a Genotypes 
653 |a Pathology 
653 |a Brain 
653 |a Cortex 
653 |a Cognition 
653 |a Encoding (Cognitive process) 
653 |a Functional magnetic resonance imaging 
653 |a Regions 
653 |a Recognition 
653 |a Older people 
653 |a Resting 
653 |a Acknowledgment 
653 |a Carriers 
653 |a Disconnection 
653 |a Changes 
653 |a Delayed memory 
653 |a Encoding 
653 |a Adults 
653 |a Functional connectivity 
653 |a Alzheimer's disease 
653 |a Memory 
653 |a Medial temporal lobe 
653 |a Sex education 
700 1 |a Adams, Jenna N.  |u University of California, Irvine, CA, USA, 
700 1 |a Molloy, Eóin N.  |u German Center for Neurodegenerative Diseases (DZNE), Magdeburg, Germany, 
700 1 |a Tremblay‐Mercier, Jennifer  |u Douglas Mental Health University Institute, Centre for Studies on the Prevention of Alzheimer's Disease (StoP‐AD), Montréal, QC, Canada, 
700 1 |a Remz, Jordana  |u Douglas Mental Health University Institute, Centre for Studies on the Prevention of Alzheimer's Disease (StoP‐AD), Montréal, QC, Canada, 
700 1 |a Binette, Alexa Pichet  |u Clinical Memory Research Unit, Department of Clinical Sciences Malmö, Lund University, MOntreal, QC, Canada, 
700 1 |a Rajah, Natasha  |u Douglas Mental Health University Institute, Centre for Studies on the Prevention of Alzheimer's Disease (StoP‐AD), Montréal, QC, Canada, 
700 1 |a Villeneuve, Sylvia  |u Department of Psychiatry, McGill University, Montréal, QC, Canada, 
700 1 |a Maass, Anne  |u German Center for Neurodegenerative Diseases (DZNE), Magdeburg, Germany, 
773 0 |t Alzheimer's & Dementia  |g vol. 21 (Dec 1, 2025) 
786 0 |d ProQuest  |t Consumer Health Database 
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