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Cell Stiffness Is a Biomarker of the Metastatic Potential of Ovarian Cancer Cells

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Yayımlandı:PLoS One vol. 7, no. 10 (Oct 2012), p. e46609
Yazar: Xu, Wenwei
Diğer Yazarlar: Mezencev, Roman, Kim, Byungkyu, Wang, Lijuan, McDonald, John, Sulchek, Todd
Baskı/Yayın Bilgisi:
Public Library of Science
Konular:
Georgia
Atlanta Georgia
United States > US
Physiology
Mechanical properties
Formability
Metastasis
Atomic structure
Disease
Stiffness
Deformability
Cytoskeleton
Atomic force microscopy
Biomedical engineering
Biology
Metastases
Kinases
Mechanical engineering
Ovarian cancer
Signal transduction
Gene expression
Social
Environmental
Blood diseases
Actin
Antibiotics
Cancer
Deformation
Microscopy
Medical research
Biomarkers
Epithelial cells
Design parameters
Invasiveness
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Özet:The metastatic potential of cells is an important parameter in the design of optimal strategies for the personalized treatment of cancer. Using atomic force microscopy (AFM), we show, consistent with previous studies conducted in other types of epithelial cancer, that ovarian cancer cells are generally softer and display lower intrinsic variability in cell stiffness than non-malignant ovarian epithelial cells. A detailed examination of highly invasive ovarian cancer cells (HEY A8) relative to their less invasive parental cells (HEY), demonstrates that deformability is also an accurate biomarker of metastatic potential. Comparative gene expression analyses indicate that the reduced stiffness of highly metastatic HEY A8 cells is associated with actin cytoskeleton remodeling and microscopic examination of actin fiber structure in these cell lines is consistent with this prediction. Our results indicate that cell stiffness may be a useful biomarker to evaluate the relative metastatic potential of ovarian and perhaps other types of cancer cells.
ISSN:1932-6203
DOI:10.1371/journal.pone.0046609
Kaynak:Health & Medical Collection