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Molecular cloning of growth hormone encoding cDNA of Indian major carps by a modified rapid amplification of cDNA ends strategy

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Publicado en:Journal of Biosciences vol. 27, no. 3 (Jun 2002), p. 261
Autor principal: Venugopal, T
Otros Autores: Mathavan, S, Pandian, T J
Publicado:
Springer Nature B.V.
Materias:
Amino Acid Sequence
Animals
Base Sequence
Carps > genetics
Cloning, Molecular
DNA Primers > genetics
DNA, Complementary > genetics
Escherichia coli > genetics
Growth Hormone > chemistry
Growth Hormone > genetics
India
Models, Molecular
Molecular Sequence Data
Nucleic Acid Amplification Techniques > methods
Pituitary Gland
Protein Conformation
Sequence Analysis, DNA
Carp
Growth hormones
Deoxyribonucleic acid > DNA
Cloning
Studies
Environmental
Acceso en línea:Citation/Abstract
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Resumen:A modified rapid amplification of cDNA ends (RACE) strategy has been developed for cloning highly conserved cDNA sequences. Using this modified method, the growth hormone (GH) encoding cDNA sequences ofLabeo rohita, Cirrhina mrigala andCatla catla have been cloned, characterized and overexpressed inEscherichia coli. These sequences show 96-98% homology to each other and are about 85% homologous to that of common carp. Besides, an attempt has been made for the first time to describe a 3-D model of the fish GH protein.[PUBLICATION ABSTRACT]   A modified rapid amplification of cDNA ends (RACE) strategy has been developed for cloning highly conserved cDNA sequences. Using this modified method, the growth hormone (GH) encoding cDNA sequences of Labeo rohita, Cirrhina mrigala and Catla catla have been cloned, characterized and overexpressed in Escherichia coli. These sequences show 96-98% homology to each other and are about 85% homologous to that of common carp. Besides, an attempt has been made for the first time to describe a 3-D model of the fish GH protein.
ISSN:0250-5991
0973-7138
DOI:10.1007/BF02704915
Fuente:Health & Medical Collection