Molecular cloning of growth hormone encoding cDNA of Indian major carps by a modified rapid amplification of cDNA ends strategy
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| Εκδόθηκε σε: | Journal of Biosciences vol. 27, no. 3 (Jun 2002), p. 261 |
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| Κύριος συγγραφέας: | |
| Άλλοι συγγραφείς: | , |
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Springer Nature B.V.
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| Διαθέσιμο Online: | Citation/Abstract Full Text Full Text - PDF |
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| 100 | 1 | |a Venugopal, T | |
| 245 | 1 | |a Molecular cloning of growth hormone encoding cDNA of Indian major carps by a modified rapid amplification of cDNA ends strategy | |
| 260 | |b Springer Nature B.V. |c Jun 2002 | ||
| 513 | |a Journal Article | ||
| 520 | 3 | |a A modified rapid amplification of cDNA ends (RACE) strategy has been developed for cloning highly conserved cDNA sequences. Using this modified method, the growth hormone (GH) encoding cDNA sequences ofLabeo rohita, Cirrhina mrigala andCatla catla have been cloned, characterized and overexpressed inEscherichia coli. These sequences show 96-98% homology to each other and are about 85% homologous to that of common carp. Besides, an attempt has been made for the first time to describe a 3-D model of the fish GH protein.[PUBLICATION ABSTRACT] A modified rapid amplification of cDNA ends (RACE) strategy has been developed for cloning highly conserved cDNA sequences. Using this modified method, the growth hormone (GH) encoding cDNA sequences of Labeo rohita, Cirrhina mrigala and Catla catla have been cloned, characterized and overexpressed in Escherichia coli. These sequences show 96-98% homology to each other and are about 85% homologous to that of common carp. Besides, an attempt has been made for the first time to describe a 3-D model of the fish GH protein. | |
| 650 | 2 | 2 | |a Amino Acid Sequence |
| 650 | 2 | 2 | |a Animals |
| 650 | 2 | 2 | |a Base Sequence |
| 650 | 1 | 2 | |a Carps |x genetics |
| 650 | 2 | 2 | |a Cloning, Molecular |
| 650 | 2 | 2 | |a DNA Primers |x genetics |
| 650 | 1 | 2 | |a DNA, Complementary |x genetics |
| 650 | 2 | 2 | |a Escherichia coli |x genetics |
| 650 | 2 | 2 | |a Growth Hormone |x chemistry |
| 650 | 1 | 2 | |a Growth Hormone |x genetics |
| 650 | 2 | 2 | |a India |
| 650 | 2 | 2 | |a Models, Molecular |
| 650 | 2 | 2 | |a Molecular Sequence Data |
| 650 | 1 | 2 | |a Nucleic Acid Amplification Techniques |x methods |
| 650 | 2 | 2 | |a Pituitary Gland |
| 650 | 2 | 2 | |a Protein Conformation |
| 650 | 2 | 2 | |a Sequence Analysis, DNA |
| 653 | |a Carp | ||
| 653 | |a Growth hormones | ||
| 653 | |a Deoxyribonucleic acid--DNA | ||
| 653 | |a Cloning | ||
| 653 | |a Studies | ||
| 653 | |a Environmental | ||
| 700 | 1 | |a Mathavan, S | |
| 700 | 1 | |a Pandian, T J | |
| 773 | 0 | |t Journal of Biosciences |g vol. 27, no. 3 (Jun 2002), p. 261 | |
| 786 | 0 | |d ProQuest |t Health & Medical Collection | |
| 856 | 4 | 1 | |3 Citation/Abstract |u https://www.proquest.com/docview/236226408/abstract/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text |u https://www.proquest.com/docview/236226408/fulltext/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text - PDF |u https://www.proquest.com/docview/236226408/fulltextPDF/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |