Imagen de Portada

Inflammation-suppressing cornea-in-a-syringe with anti-viral GF19 peptide promotes regeneration in HSV-1 infected rabbit corneas

Guardado en:
Publicado en:NPJ Regenerative Medicine vol. 9, no. 1 (2024), p. 11
Autor principal: Simoliunas, Egidijus
Otros Autores: Ruedas-Torres, Inés, Jiménez-Gómez, Yolanda, Edin, Elle, Aghajanzadeh-Kiyaseh, Mozhgan, Zamani-Roudbaraki, Mostafa, Asoklis, Rimvydas, Alksne, Milda, Thathapudi, Neethi C., Poudel, Bijay K., Rinkunaite, Ieva, Asoklis, Kasparas, Iesmantaite, Monika, Ortega-Llamas, Laura, Makselis, Almantas, Munoz, Marcelo, Baltriukiene, Daiva, Bukelskiene, Virginija, Gómez-Laguna, Jaime, González-Andrades, Miguel, Griffith, May
Publicado:
Nature Publishing Group
Materias:
Infections
Corneal transplantation
Collagen
Nanoparticles
Regenerative medicine
Polymerization
Herpes viruses
Hospitals
Amino acids
Inflammation
Peptides
Viral infections
Polyethylene glycol
Hydrogels
Antiviral drugs
Acceso en línea:Citation/Abstract
Full Text - PDF
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
Resumen:Pathophysiologic inflammation, e.g., from HSV-1 viral infection, can cause tissue destruction resulting in ulceration, perforation, and ultimately blindness. We developed an injectable Cornea-in-a-Syringe (CIS) sealant-filler to treat damaged corneas. CIS comprises linear carboxylated polymers of inflammation-suppressing 2-methacryloyloxyethyl phosphorylcholine, regeneration-promoting collagen-like peptide, and adhesive collagen-citrate glue. We also incorporated GF19, a modified anti-viral host defense peptide that blocked HSV-1 activity in vitro when released from silica nanoparticles (SiNP-GF19). CIS alone suppressed inflammation when tested in a surgically perforated and HSV-1-infected rabbit corneal model, allowing tissue and nerve regeneration. However, at six months post-operation, only regenerated neocorneas previously treated with CIS with SiNP-GF19 had structural and functional features approaching those of normal healthy corneas and were HSV-1 virus-free. We showed that composite injectable biomaterials can be designed to allow regeneration by modulating inflammation and blocking viral activity in an infected tissue. Future iterations could be optimized for clinical application.
ISSN:2057-3995
DOI:10.1038/s41536-024-00355-1
Fuente:Health & Medical Collection