microRNA Profile of High-Grade B-Cell Lymphoma with 11q Aberration

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Publicado en:International Journal of Molecular Sciences vol. 26, no. 1 (2025), p. 285
Autor principal: Zajdel, Michalina
Otros Autores: Szafron, Łukasz Michał, Paziewska, Agnieszka, Rymkiewicz, Grzegorz, Dąbrowska, Michalina, Bystydzieński, Zbigniew, Kulińczak, Mariusz, Grygalewicz, Beata, Sromek, Maria, Błachnio, Katarzyna, Kulecka, Maria, Hajdyła, Filip, Goryca, Krzysztof, Chechlińska, Magdalena, Jan Konrad Siwicki
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MDPI AG
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Resumen:High-grade B-cell lymphoma with 11q aberration (HGBCL-11q) is a rare germi-nal centre lymphoma characterised by a typical gain/loss pattern on chromo-some 11q but without MYC translocation. It shares some features with Burkitt lymphoma (BL), HGBCLs and germinal centre-derived diffuse large B-cell lym-phoma, not otherwise specified (GCB-DLBCL-NOS). Since microRNA expression in HGBCL-11q remains unknown, we aimed to identify and compare the mi-croRNA expression profiles in HGBCL-11q, BL and in GCB-DLBCL-NOS. Next-generation sequencing (NGS)-based microRNA profiling of HGBCL-11q (n = 6), BL (n = 8), and GCB-DLBCL-NOS without (n = 3) and with MYC rearrange-ment (MYC-R) (n = 7) was performed. We identified sets of 39, 64, and 49 mi-croRNAs differentiating HGBCL-11q from BL, and from GCB-DLBCL-NOS without MYC-R, respectively. The expression levels of miR-223-3p, miR-193b-3p, miR-29b-3p, and miR-146a-5p consistently differentiated HGBCL-11q from both BL, GCB-DLBCL-NOS without MYC-R. In addition, HGBCL-11q presented greater heterogeneity in microRNA expression than BL. The expression profile of MYC-regulated microRNAs differed in HGBCL-11q and in BL, while also clearly distinguishing HGBCL-11q and BL from GCB-DLBCL-NOS. The microRNA pro-file of HGBCL-11q differs from those of BL and GCB-DLBCL-NOS, exhibiting greater heterogeneity compared to BL. The microRNA profile further supports that HGBCL-11q is a distinct subtype of B-cell lymphoma.
ISSN:1661-6596
1422-0067
DOI:10.3390/ijms26010285
Fuente:Health & Medical Collection