A major update of the genome assembly of Eri silkmoth, Samia ricini.
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| Udgivet i: | bioRxiv (Jan 14, 2025) |
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| Hovedforfatter: | |
| Andre forfattere: | , , |
| Udgivet: |
Cold Spring Harbor Laboratory Press
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| Online adgang: | Citation/Abstract Full text outside of ProQuest |
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| Resumen: | Indian eri silkmoth, Samia ricini, is a wild silkmoth whose silk occupies a significant economic position. In addition to its importance as an economic animal, S. ricini is also useful as a model species of Saturniidae. National BioResource of Japan (NBRP) maintains a S. ricini strain brought to Japan during WWII via Taiwan. Since we have previously published a draft genome assembly of S. ricini, we have attempted to construct a chromosome-level genome assembly to facilitate genetic studies of S. ricini. We successfully constructed a chromosome-scale genome assembly by exploiting two long-read-based technologies, HiFi reads and optical genome mapping. Furthermore, we performed functional annotations of the genome assembly, i.e., repeat annotation, transcriptome-based gene prediction, ATAC-seq, and PIWI-interacting RNA (piRNA)-targeted small RNA-seq. The assembly harbours 16,226 protein-coding genes and 636 piRNA clusters across three tissues: ovaries, testis, and embryos. ATAC-seq data comprehensively detected open chromosome regions, which will benefit when CRISPR/Cas9-mediated genome editing is conducted.Competing Interest StatementThe authors have declared no competing interest.Footnotes* Citations for nucleotide sequences (AP038896 to AP038909) were added.* https://figshare.com/articles/dataset/The_genome_annotation_files_of_the_latest_genome_assembly_of_Indian_eri_silkmoth_i_Samia_ricini_i_including_gene_models_its_functional_annotation_result_and_piRNA_cluster_maps_/27268212 |
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| ISSN: | 2692-8205 |
| DOI: | 10.1101/2025.01.09.632291 |
| Fuente: | Biological Science Database |