A major update of the genome assembly of Eri silkmoth, Samia ricini.
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| Publicado no: | bioRxiv (Jan 14, 2025) |
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Cold Spring Harbor Laboratory Press
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| Acesso em linha: | Citation/Abstract Full text outside of ProQuest |
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| 024 | 7 | |a 10.1101/2025.01.09.632291 |2 doi | |
| 035 | |a 3154281138 | ||
| 045 | 0 | |b d20250114 | |
| 100 | 1 | |a Jung, Lee | |
| 245 | 1 | |a A major update of the genome assembly of Eri silkmoth, Samia ricini. | |
| 260 | |b Cold Spring Harbor Laboratory Press |c Jan 14, 2025 | ||
| 513 | |a Working Paper | ||
| 520 | 3 | |a Indian eri silkmoth, Samia ricini, is a wild silkmoth whose silk occupies a significant economic position. In addition to its importance as an economic animal, S. ricini is also useful as a model species of Saturniidae. National BioResource of Japan (NBRP) maintains a S. ricini strain brought to Japan during WWII via Taiwan. Since we have previously published a draft genome assembly of S. ricini, we have attempted to construct a chromosome-level genome assembly to facilitate genetic studies of S. ricini. We successfully constructed a chromosome-scale genome assembly by exploiting two long-read-based technologies, HiFi reads and optical genome mapping. Furthermore, we performed functional annotations of the genome assembly, i.e., repeat annotation, transcriptome-based gene prediction, ATAC-seq, and PIWI-interacting RNA (piRNA)-targeted small RNA-seq. The assembly harbours 16,226 protein-coding genes and 636 piRNA clusters across three tissues: ovaries, testis, and embryos. ATAC-seq data comprehensively detected open chromosome regions, which will benefit when CRISPR/Cas9-mediated genome editing is conducted.Competing Interest StatementThe authors have declared no competing interest.Footnotes* Citations for nucleotide sequences (AP038896 to AP038909) were added.* https://figshare.com/articles/dataset/The_genome_annotation_files_of_the_latest_genome_assembly_of_Indian_eri_silkmoth_i_Samia_ricini_i_including_gene_models_its_functional_annotation_result_and_piRNA_cluster_maps_/27268212 | |
| 651 | 4 | |a Japan | |
| 653 | |a Chromosomes | ||
| 653 | |a Transcriptomes | ||
| 653 | |a Embryos | ||
| 653 | |a Genomes | ||
| 653 | |a CRISPR | ||
| 653 | |a Gene mapping | ||
| 653 | |a Samia cynthia ricini | ||
| 700 | 1 | |a Okamoto, Mana | |
| 700 | 1 | |a Kawagoe, Rin | |
| 700 | 1 | |a Shimada, Toru | |
| 773 | 0 | |t bioRxiv |g (Jan 14, 2025) | |
| 786 | 0 | |d ProQuest |t Biological Science Database | |
| 856 | 4 | 1 | |3 Citation/Abstract |u https://www.proquest.com/docview/3154281138/abstract/embedded/L8HZQI7Z43R0LA5T?source=fedsrch |
| 856 | 4 | 0 | |3 Full text outside of ProQuest |u https://www.biorxiv.org/content/10.1101/2025.01.09.632291v2 |