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Candidate transmission survival genome of Mycobacterium tuberculosis

Guardado en:
Udgivet i:bioRxiv (Jan 30, 2025)
Hovedforfatter: Mishra, Saurabh
Andre forfattere: Singh, Prabhat Ranjan, Hu, Xiaoyi, Lopez-Quezada, Landys, Jinich, Adrian, Jahn, Robin, Geurts, Luc, Shen, Naijian, Dejesus, Michael, Hartman, Travis, Kyu Young Rhee, Zimmerman, Matthew, Dartois, Veronique A, Jones, Richard M, Jiang, Xiuju, Almada-Monter, Ricardo, Bourouiba, Lydia, Nathan, Carl F
Udgivet:
Cold Spring Harbor Laboratory Press
Fag:
Transcriptomes
Genomes
Drug tolerance
Atmospheric conditions
Tuberculosis
Cell survival
Mycobacterium tuberculosis
Online adgang:Citation/Abstract
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Resumen:Mycobacterium tuberculosis (Mtb), a leading cause of death from infection, completes its life cycle entirely in humans except for transmission through the air. To begin to understand how Mtb survives aerosolization, we mimicked liquid and atmospheric conditions experienced by Mtb before and after exhalation using a model aerosol fluid (MAF) based on the water-soluble, lipidic and cellular constituents of necrotic tuberculosis lesions. MAF induced drug tolerance in Mtb, remodeled its transcriptome and protected Mtb from dying in microdroplets desiccating in air. Yet survival was not passive: Mtb appeared to rely on hundreds of genes to survive conditions associated with transmission. Essential genes subserving proteostasis offered most protection. A large number of conventionally nonessential genes appeared to contribute as well, including genes encoding proteins that resemble anti-desiccants. The candidate transmission survival genome of Mtb may offer opportunities to reduce transmission of tuberculosis.Competing Interest StatementThe authors have declared no competing interest.Footnotes* https://doi.org/10.7298/e4xs-y663
ISSN:2692-8205
DOI:10.1101/2025.01.30.635747
Fuente:Biological Science Database