Candidate transmission survival genome of Mycobacterium tuberculosis

محفوظ في:

التفاصيل البيبلوغرافية
الحاوية / القاعدة:	bioRxiv (Jan 30, 2025)
المؤلف الرئيسي:	Mishra, Saurabh
مؤلفون آخرون:	Singh, Prabhat Ranjan, Hu, Xiaoyi, Lopez-Quezada, Landys, Jinich, Adrian, Jahn, Robin, Geurts, Luc, Shen, Naijian, Dejesus, Michael, Hartman, Travis, Kyu Young Rhee, Zimmerman, Matthew, Dartois, Veronique A, Jones, Richard M, Jiang, Xiuju, Almada-Monter, Ricardo, Bourouiba, Lydia, Nathan, Carl F
منشور في:	Cold Spring Harbor Laboratory Press
الموضوعات:	Transcriptomes Genomes Drug tolerance Atmospheric conditions Tuberculosis Cell survival Mycobacterium tuberculosis
الوصول للمادة أونلاين:	Citation/Abstract Full text outside of ProQuest
الوسوم:	إضافة وسم لا توجد وسوم, كن أول من يضع وسما على هذه التسجيلة!

الوصف
مستخلص:	Mycobacterium tuberculosis (Mtb), a leading cause of death from infection, completes its life cycle entirely in humans except for transmission through the air. To begin to understand how Mtb survives aerosolization, we mimicked liquid and atmospheric conditions experienced by Mtb before and after exhalation using a model aerosol fluid (MAF) based on the water-soluble, lipidic and cellular constituents of necrotic tuberculosis lesions. MAF induced drug tolerance in Mtb, remodeled its transcriptome and protected Mtb from dying in microdroplets desiccating in air. Yet survival was not passive: Mtb appeared to rely on hundreds of genes to survive conditions associated with transmission. Essential genes subserving proteostasis offered most protection. A large number of conventionally nonessential genes appeared to contribute as well, including genes encoding proteins that resemble anti-desiccants. The candidate transmission survival genome of Mtb may offer opportunities to reduce transmission of tuberculosis.Competing Interest StatementThe authors have declared no competing interest.Footnotes* https://doi.org/10.7298/e4xs-y663
تدمد:	2692-8205
DOI:	10.1101/2025.01.30.635747
المصدر:	Biological Science Database