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Profiling gene alterations in striatonigral neurons associated with incubation of methamphetamine craving by cholera toxin subunit B-based fluorescence-activated cell sorting

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Publicado en:Frontiers in Cellular Neuroscience (Feb 12, 2025)
Autor principal: Altshuler, Rachel D
Otros Autores: Burke, Megan A M, Garcia, Kristine T, Class, Kenneth, Cimbro, Raffaello, Li, Xuan
Publicado:
Frontiers Research Foundation
Materias:
University of Maryland
United States > US
Behavior
Cholera toxin
Catheters
Caudate-putamen
Molecular modelling
Spiny neurons
Flow cytometry
Genes
Neostriatum
Laboratory animals
Narcotics
Proteins
Fluorescence
Abstinence
Polyethylene
Dopamine
Surgery
FDA approval
Experiments
Methamphetamine
Cholera
Enzymes
Toxins
Roles
Neurosciences
Acceso en línea:Citation/Abstract
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Resumen:Introduction: In both rats and humans, methamphetamine (Meth) seeking progressively increases during abstinence, a behavioral phenomenon termed “incubation of Meth craving”. We previously demonstrated a critical role of dorsal striatum (DS) in this incubation in rats. However, circuit-specific molecular mechanisms in DS underlying this incubation are largely unknown. Here we combined a newly developed fluorescence-activated sorting (FACS) protocol with fluorescence-conjugated cholera toxin subunit B-647 (CTb-647, a retrograde tracer) to examine gene alterations in the direct-pathway (striatonigral) medium spiny neurons (MSNs) associated with incubation of Meth craving.
ISSN:1662-5102
DOI:10.3389/fncel.2025.1542508
Fuente:Science Database