Profiling gene alterations in striatonigral neurons associated with incubation of methamphetamine craving by cholera toxin subunit B-based fluorescence-activated cell sorting

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Publicat a:Frontiers in Cellular Neuroscience (Feb 12, 2025)
Autor principal: Altshuler, Rachel D
Altres autors: Burke, Megan A M, Garcia, Kristine T, Class, Kenneth, Cimbro, Raffaello, Li, Xuan
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Frontiers Research Foundation
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LEADER 00000nab a2200000uu 4500
001 3165948050
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022 |a 1662-5102 
024 7 |a 10.3389/fncel.2025.1542508  |2 doi 
035 |a 3165948050 
045 0 |b d20250212 
100 1 |a Altshuler, Rachel D 
245 1 |a Profiling gene alterations in striatonigral neurons associated with incubation of methamphetamine craving by cholera toxin subunit B-based fluorescence-activated cell sorting 
260 |b Frontiers Research Foundation  |c Feb 12, 2025 
513 |a Journal Article 
520 3 |a Introduction: In both rats and humans, methamphetamine (Meth) seeking progressively increases during abstinence, a behavioral phenomenon termed “incubation of Meth craving”. We previously demonstrated a critical role of dorsal striatum (DS) in this incubation in rats. However, circuit-specific molecular mechanisms in DS underlying this incubation are largely unknown. Here we combined a newly developed fluorescence-activated sorting (FACS) protocol with fluorescence-conjugated cholera toxin subunit B-647 (CTb-647, a retrograde tracer) to examine gene alterations in the direct-pathway (striatonigral) medium spiny neurons (MSNs) associated with incubation of Meth craving. 
610 4 |a University of Maryland 
651 4 |a United States--US 
653 |a Behavior 
653 |a Cholera toxin 
653 |a Catheters 
653 |a Caudate-putamen 
653 |a Molecular modelling 
653 |a Spiny neurons 
653 |a Flow cytometry 
653 |a Genes 
653 |a Neostriatum 
653 |a Laboratory animals 
653 |a Narcotics 
653 |a Proteins 
653 |a Fluorescence 
653 |a Abstinence 
653 |a Polyethylene 
653 |a Dopamine 
653 |a Surgery 
653 |a FDA approval 
653 |a Experiments 
653 |a Methamphetamine 
653 |a Cholera 
653 |a Enzymes 
653 |a Toxins 
653 |a Roles 
653 |a Neurosciences 
700 1 |a Burke, Megan A M 
700 1 |a Garcia, Kristine T 
700 1 |a Class, Kenneth 
700 1 |a Cimbro, Raffaello 
700 1 |a Li, Xuan 
773 0 |t Frontiers in Cellular Neuroscience  |g (Feb 12, 2025) 
786 0 |d ProQuest  |t Science Database 
856 4 1 |3 Citation/Abstract  |u https://www.proquest.com/docview/3165948050/abstract/embedded/L8HZQI7Z43R0LA5T?source=fedsrch 
856 4 0 |3 Full Text + Graphics  |u https://www.proquest.com/docview/3165948050/fulltextwithgraphics/embedded/L8HZQI7Z43R0LA5T?source=fedsrch 
856 4 0 |3 Full Text - PDF  |u https://www.proquest.com/docview/3165948050/fulltextPDF/embedded/L8HZQI7Z43R0LA5T?source=fedsrch