Buchumschlag

Production of an Innovative, Surface Area‐Enhanced and Biodegradable Biofilm‐Generating Device by 3D Printing

Gespeichert in:
Veröffentlicht in:Engineering in Life Sciences vol. 25, no. 2 (Feb 1, 2025)
1. Verfasser: Datta, Atulona
Weitere Verfasser: Saha, Rituparna, Sahoo, Sovan, Roy, Arup Ratan, Basu, Shayontani, Mahajan, Girish, Panja, Subhash Chandra, Mukherjee, Joydeep
Veröffentlicht:
John Wiley & Sons, Inc.
Schlagworte:
Jadavpur University
India
Software
Fused deposition modeling
Accessibility
Transplants & implants
Klebsiella
pH
Surface area
Biofilms
Water treatment
Scanning microscopy
Design specifications
Confocal microscopy
Metabolites
Biodegradability
Polylactic acid
Three dimensional printing
Visualization
Polymers
Dissolved gases
Computer aided design > CAD
Medical research
3-D printers
E coli
Shipping industry
Online-Zugang:Citation/Abstract
Full Text
Full Text - PDF
Tags: Tag hinzufügen
Keine Tags, Fügen Sie das erste Tag hinzu!
Abstract:ABSTRACT The enhanced surface cylindrical flask (ESCF) consists of an eight‐striped inner arrangement holding 16 standard microscopic slides placed inside a cylindrical vessel. The specially designed spatula‐accessible slides can be withdrawn from the vessel during cultivation without disturbing biofilm formation through an innovative window‐flap accessibility mechanism. The vessel and its accessories were three‐dimensional (3D) printed by applying a fused deposition modeling technique utilizing biodegradable polylactic acid. Biofilms of clinically relevant bacteria namely Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli were successfully grown in the ESCF and observed through confocal laser scanning microscopy. Advantages of the device include an enhanced surface area for biofilm formation, ease of insertion and removal of microscopic slides, convenient fitting into standard rotary shaker platforms, creation of anoxic/microaerophilic environment inside the vessel as well as the feasibility of pH, dissolved gases, and metabolite measurements in the liquid surrounding the biofilm. The ESCF will find widespread application in medical, industrial, and environmental disciplines.
ISSN:1618-2863
DOI:10.1002/elsc.202400046
Quelle:Engineering Database