Beeld op de omslag

Harnessing advanced computational approaches to design novel antimicrobial peptides against intracellular bacterial infections

Bewaard in:
Gepubliceerd in:Bioactive Materials vol. 50 (2025), p. 510
Hoofdauteur: Fang, Yanpeng
Andere auteurs: Fan, Duoyang, Feng, Bin, Zhu, Yingli, Xie, Ruyan, Tan, Xiaorong, Liu, Qianhui, Dong, Jie, Zeng, Wenbin
Gepubliceerd in:
KeAi Publishing Communications Ltd
Onderwerpen:
Biocompatibility
Databases
Cytotoxicity
Pathogens
Datasets
Toxicity
Drug resistance
Bacteria
Staphylococcus infections
Drug development
Amino acids
Vancomycin
Computer applications
Peptides
Prediction models
Efficiency
Therapeutic applications
Membranes
Simulation
Artificial intelligence
Cell membranes
Antibiotics
Bacterial infections
Antimicrobial agents
Antimicrobial peptides
Intracellular
Tuberculosis
Antimicrobial activity
Online toegang:Citation/Abstract
Full Text
Full Text - PDF
Tags: Voeg label toe
Geen labels, Wees de eerste die dit record labelt!
Samenvatting:Intracellular bacterial infections pose a significant challenge to current therapeutic strategies due to the limited penetration of antibiotics through host cell membranes. This study presents a novel computational framework for efficiently screening candidate peptides against these infections. The proposed strategy comprehensively evaluates the essential properties for the clinical application of candidate peptides, including antimicrobial activity, permeation efficiency, and biocompatibility, while also taking into account the speed and reliability of the screening process. A combination of multiple AI-based activity prediction models allows for a thorough assessment of sequences in the cell-penetrating peptides (CPPs) database and quickly identifies candidate peptides with target properties. On this basis, the CPP microscopic dynamics research system was constructed. Exploration of the mechanism of action at the atomic level provides strong support for the discovery of promising candidate peptides. Promising candidates are subsequently validated through in vitro and in vivo experiments. Finally, Crot-1 was rapidly identified from the CPPsite 2.0 database. Crot-1 effectively eradicated intracellular MRSA, demonstrating significantly greater efficacy than vancomycin. Moreover, it exhibited no apparent cytotoxicity to host cells, highlighting its potential for clinical application. This work offers a promising new avenue for developing novel antimicrobial materials to combat intracellular bacterial infections.
ISSN:2097-1192
2452-199X
DOI:10.1016/j.bioactmat.2025.04.016
Bron:Materials Science Database