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Computational design and evaluation of optimal bait sets for scalable proximity proteomics

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Publicado en:Nature Communications vol. 16, no. 1 (2025), p. 9333-9350
Autor principal: Kasmaeifar, Vesal
Otros Autores: Sedighi, Saya, Gingras, Anne-Claude, Campbell, Kieran R.
Publicado:
Nature Publishing Group
Materias:
Datasets
Proteomics
Mutation
Labeling
Baits
Feature selection
Proximity
Data analysis
Computer applications
Maps
Biological activity
Localization
Proteins
Machine learning
Peptide mapping
Spatial data
Genetic algorithms
Proteomes
Biotinylation
Enzymes
Software
Environmental
Acceso en línea:Citation/Abstract
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Resumen:The spatial organization of proteins within eukaryotic cells underlies essential biological processes and can be mapped by identifying nearby proteins using proximity-dependent biotinylation approaches such as BioID. When applied systematically to hundreds of bait proteins, BioID has localized thousands of endogenous proteins in human cells, generating a comprehensive view of subcellular organization. However, the need for large bait sets limits the scalability of BioID for context-dependent spatial profiling across different cell types, states, or perturbations. To address this, we develop a benchmarking framework with multiple complementary metrics to assess how well a given bait subset recapitulates the structure and coverage of a reference BioID dataset. We also introduce GENBAIT, a genetic algorithm-based method that identifies optimized bait subsets predicted to retain maximal spatial information while reducing the total number of baits. Applied to three large BioID datasets, GENBAIT consistently selected subsets representing less than one-third of the original baits while preserving high coverage and network integrity. This flexible, data-driven approach enables intelligent bait selection for targeted, context-specific studies, thereby expanding the accessibility of large-scale subcellular proteome mapping.Proximity-dependent biotinylation maps protein locations using multiple bait proteins, limiting scalability. Here, the authors present GENBAIT, a computational tool that selects optimal baits to reduce the number of experiments required while preserving subcellular organization.
ISSN:2041-1723
DOI:10.1038/s41467-025-64383-1
Fuente:Health & Medical Collection