Imagen de Portada

Populus PtrbHLH011 Is a Transcriptional Co‐Regulator Involved in the Activation of Cell Wall Biosynthesis by Iron Deprivation

Guardado en:
Publicado en:Plant Biotechnology Journal vol. 23, no. 11 (Nov 1, 2025), p. 4893-4910
Autor principal: Tadesse, Dimiru
Otros Autores: Dai, Yuqiu, Li, Gen, Yang, Lin, Yang, Yang, Dwivedi, Nidhi, Kumaran, Desigan, Blaby‐Haas, Crysten E., Lipzen, Anna, Santiago, Kassandra, Barry, Kerrie, Coleman, Gary, Qi, Yiping, Liu, Chang‐Jun, Xie, Meng
Publicado:
John Wiley & Sons, Inc.
Materias:
Lignocellulose
Biosynthesis
Flavonoids
Deprivation
Lignin
Iron
Cell walls
Energy crops
Biomass
Homeostasis
Renewable energy
Plant growth
Genes
Cell activation
Functionals
Cytosine
Cellulose
Iron deficiency
Stems
Target recognition
Regulatory mechanisms (biology)
Environmental
Acceso en línea:Citation/Abstract
Full Text
Full Text - PDF
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
Resumen:ABSTRACT The lack of a mechanistic understanding of the environmental plasticity of secondary cell wall (SCW) biosynthesis restricts large‐scale biomass and bioenergy production on marginal lands. Using Populus (poplar), a key bioenergy crop, we discovered that iron deprivation, a prevalent abiotic stress on marginal lands, stimulates SCW biosynthesis in stems. We identified the transcription factor PtrbHLH011 as a critical regulator underlying this response. Through integrated analyses involving phenotypic characterisation of PtrbHLH011 knockout and overexpression plants, functional genomics and molecular investigations, we established that PtrbHLH011 functions as a central regulator of SCW biosynthesis, iron homeostasis and flavonoid biosynthesis by directly repressing essential genes in these pathways. Iron deprivation downregulates PtrbHLH011 expression, subsequently activating these biosynthetic pathways. Notably, cytosine base editing‐based knockout of PtrbHLH011 significantly enhanced plant growth, yielding up to a 110% increase in stem diameter and a 300% increase in leaf iron content. These findings present a novel regulatory mechanism linking environmental iron availability to SCW biosynthesis and illustrate a practical strategy to improve biomass yield on iron‐deficient marginal lands. Furthermore, our mechanistic insights into PtrbHLH011 target recognition and regulation provide a valuable foundation for precise manipulation of gene regulatory networks, facilitating the development of high‐performance bioenergy crops adapted to marginal environments.
ISSN:1467-7644
1467-7652
DOI:10.1111/pbi.70275
Fuente:Engineering Database