Populus PtrbHLH011 Is a Transcriptional Co‐Regulator Involved in the Activation of Cell Wall Biosynthesis by Iron Deprivation
Wedi'i Gadw mewn:
| Cyhoeddwyd yn: | Plant Biotechnology Journal vol. 23, no. 11 (Nov 1, 2025), p. 4893-4910 |
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| Prif Awdur: | |
| Awduron Eraill: | , , , , , , , , , , , , , |
| Cyhoeddwyd: |
John Wiley & Sons, Inc.
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| Pynciau: | |
| Mynediad Ar-lein: | Citation/Abstract Full Text Full Text - PDF |
| Tagiau: |
Dim Tagiau, Byddwch y cyntaf i dagio'r cofnod hwn!
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MARC
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| 022 | |a 1467-7644 | ||
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| 024 | 7 | |a 10.1111/pbi.70275 |2 doi | |
| 035 | |a 3267232486 | ||
| 045 | 0 | |b d20251101 | |
| 084 | |a 168442 |2 nlm | ||
| 100 | 1 | |a Tadesse, Dimiru |u Biology Department, Brookhaven National Laboratory, Upton, New York, USA | |
| 245 | 1 | |a Populus PtrbHLH011 Is a Transcriptional Co‐Regulator Involved in the Activation of Cell Wall Biosynthesis by Iron Deprivation | |
| 260 | |b John Wiley & Sons, Inc. |c Nov 1, 2025 | ||
| 513 | |a Journal Article | ||
| 520 | 3 | |a ABSTRACT The lack of a mechanistic understanding of the environmental plasticity of secondary cell wall (SCW) biosynthesis restricts large‐scale biomass and bioenergy production on marginal lands. Using Populus (poplar), a key bioenergy crop, we discovered that iron deprivation, a prevalent abiotic stress on marginal lands, stimulates SCW biosynthesis in stems. We identified the transcription factor PtrbHLH011 as a critical regulator underlying this response. Through integrated analyses involving phenotypic characterisation of PtrbHLH011 knockout and overexpression plants, functional genomics and molecular investigations, we established that PtrbHLH011 functions as a central regulator of SCW biosynthesis, iron homeostasis and flavonoid biosynthesis by directly repressing essential genes in these pathways. Iron deprivation downregulates PtrbHLH011 expression, subsequently activating these biosynthetic pathways. Notably, cytosine base editing‐based knockout of PtrbHLH011 significantly enhanced plant growth, yielding up to a 110% increase in stem diameter and a 300% increase in leaf iron content. These findings present a novel regulatory mechanism linking environmental iron availability to SCW biosynthesis and illustrate a practical strategy to improve biomass yield on iron‐deficient marginal lands. Furthermore, our mechanistic insights into PtrbHLH011 target recognition and regulation provide a valuable foundation for precise manipulation of gene regulatory networks, facilitating the development of high‐performance bioenergy crops adapted to marginal environments. | |
| 653 | |a Lignocellulose | ||
| 653 | |a Biosynthesis | ||
| 653 | |a Flavonoids | ||
| 653 | |a Deprivation | ||
| 653 | |a Lignin | ||
| 653 | |a Iron | ||
| 653 | |a Cell walls | ||
| 653 | |a Energy crops | ||
| 653 | |a Biomass | ||
| 653 | |a Homeostasis | ||
| 653 | |a Renewable energy | ||
| 653 | |a Plant growth | ||
| 653 | |a Genes | ||
| 653 | |a Cell activation | ||
| 653 | |a Functionals | ||
| 653 | |a Cytosine | ||
| 653 | |a Cellulose | ||
| 653 | |a Iron deficiency | ||
| 653 | |a Stems | ||
| 653 | |a Target recognition | ||
| 653 | |a Regulatory mechanisms (biology) | ||
| 653 | |a Environmental | ||
| 700 | 1 | |a Dai, Yuqiu |u Biology Department, Brookhaven National Laboratory, Upton, New York, USA | |
| 700 | 1 | |a Li, Gen |u Department of Plant Science and Landscape Architecture, University of Maryland, College Park, Maryland, USA | |
| 700 | 1 | |a Yang, Lin |u National Synchrotron Light Source, Brookhaven National Laboratory, New York, New York, USA | |
| 700 | 1 | |a Yang, Yang |u National Synchrotron Light Source, Brookhaven National Laboratory, New York, New York, USA | |
| 700 | 1 | |a Dwivedi, Nidhi |u Biology Department, Brookhaven National Laboratory, Upton, New York, USA | |
| 700 | 1 | |a Kumaran, Desigan |u Biology Department, Brookhaven National Laboratory, Upton, New York, USA | |
| 700 | 1 | |a Blaby‐Haas, Crysten E. |u Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, California, USA | |
| 700 | 1 | |a Lipzen, Anna |u Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, California, USA | |
| 700 | 1 | |a Santiago, Kassandra |u Biology Department, Brookhaven National Laboratory, Upton, New York, USA | |
| 700 | 1 | |a Barry, Kerrie |u Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, California, USA | |
| 700 | 1 | |a Coleman, Gary |u Department of Plant Science and Landscape Architecture, University of Maryland, College Park, Maryland, USA | |
| 700 | 1 | |a Qi, Yiping |u Department of Plant Science and Landscape Architecture, University of Maryland, College Park, Maryland, USA | |
| 700 | 1 | |a Liu, Chang‐Jun |u Biology Department, Brookhaven National Laboratory, Upton, New York, USA | |
| 700 | 1 | |a Xie, Meng |u Biology Department, Brookhaven National Laboratory, Upton, New York, USA | |
| 773 | 0 | |t Plant Biotechnology Journal |g vol. 23, no. 11 (Nov 1, 2025), p. 4893-4910 | |
| 786 | 0 | |d ProQuest |t Engineering Database | |
| 856 | 4 | 1 | |3 Citation/Abstract |u https://www.proquest.com/docview/3267232486/abstract/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text |u https://www.proquest.com/docview/3267232486/fulltext/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text - PDF |u https://www.proquest.com/docview/3267232486/fulltextPDF/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |