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Temporal sampling of root exudates using coated blade-SPME for decoding plant–pathogen interactions

Tallennettuna:
Julkaisussa:Frontiers in Plant Science vol. 16 (Sep 2025), p. 1616881-1616891
Päätekijä: Halane, Hodan
Muut tekijät: McDowell, Tim, Dhaubhadel, Sangeeta
Julkaistu:
Frontiers Media SA
Aiheet:
Pathogens
Soil ecology
Sampling techniques
Sampling methods
Metabolomics
Seeds
Rhizosphere
Natural environment
Exudates
Metabolites
Methods
Sampling
Hydroponics
Research & development > R&D
Roots
Exudation
Solid phases
Solid phase methods
Microbiomes
Environmental
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Abstrakti:Root exudates shape the rhizosphere and mediate plant–microbe interactions, yet traditional sampling techniques often disturb the natural environment. Here, we present the use of coated blade-solid-phase microextraction (CB-SPME) method for passive, non-invasive in situ temporal metabolomic profiling of pea ( Pisum sativum ) root exudates during infection by the soil-borne pathogen Aphanomyces euteiches. In comparison to previously established extraction techniques, CB-SPME delivered lower absolute recovery but superior reproducibility while maintaining sensitivity. This non-destructive approach preserves rhizosphere integrity, enabling continuous monitoring of dynamic metabolite fluctuations and offers new insights into how root exudate influences plant–microbiome interactions.
ISSN:1664-462X
DOI:10.3389/fpls.2025.1616881
Lähde:Agriculture Science Database