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NGS-based approach for diagnostically unidentified Mycobacterium saskatchewanense, a rare non-tuberculous mycobacterium

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Publicado en:Frontiers in Cellular and Infection Microbiology vol. 15 (Dec 2025), p. 1685898-1685909
Autor principal: Gatti, Giulia
Otros Autores: Ingletto, Ludovica, Dirani, Giorgio, Zannoli, Silvia, Taddei, Francesca, Colosimo, Claudia, Dionisi, Laura, Marzucco, Anna, Montanari, Maria Sofia, Denicolò, Agnese, Congestrì, Francesco, Grumiro, Laura, Brandolini, Martina, Guerra, Massimiliano, Alessandra Mistral De Pascali, Scagliarini, Alessandra, Cricca, Monica, Sambri, Vittorio
Publicado:
Frontiers Media SA
Materias:
Illumina Inc
United States > US
Germany
Infections
Software
RpoB protein
Pathogens
Phylogenetics
Heat shock proteins
Identification
Principal components analysis
Mutation
Leprosy
Nucleotide sequence
Genes
Genotype & phenotype
Drug resistance
Hsp65 protein
Next-generation sequencing
Tuberculosis
Maximum likelihood method
Whole genome sequencing
Genomic analysis
Single-nucleotide polymorphism
Multilocus sequence typing
Mycobacterium
Acceso en línea:Citation/Abstract
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Resumen:IntroductionThe implementation of advanced technologies and algorithms for diagnosis and genome analysis has made a fundamental contribution to pathogens’ identification and investigation.MethodsThe study of non-tuberculous mycobacteria (NTM) benefited from a next-generation sequencing (NGS) approach, making it possible to describe sequences of rare pathogens. This study identified 20 diagnostically unknown isolates as Mycobacterium saskatchewanense ST 691, an environmental NTM. The isolates were sequenced on two different platforms to compare their throughput and to investigate shared and unique single nucleotide polymorphism (SNP) counts, phylogeny based on concatenated 16S, hsp65, and rpoB genes, and core-genome multilocus sequence typing (MLST), in order to broaden the current knowledge of Mycobacterium saskatchewanense .ResultsPrincipal component analysis on the three genes combined with the mutations’ annotation suggests that rpoB may serve as a suitable marker to distinguish M. saskatchewanense from other NTM.DiscussionOur results show that frontier studies performed using NGS can help in overcoming the limits of traditional diagnostic assays and deepen the knowledge on rare and uncommon NTM that are raising clinical concern.
ISSN:2235-2988
DOI:10.3389/fcimb.2025.1685898
Fuente:Public Health Database