NGS-based approach for diagnostically unidentified Mycobacterium saskatchewanense, a rare non-tuberculous mycobacterium
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| Publicado en: | Frontiers in Cellular and Infection Microbiology vol. 15 (Dec 2025), p. 1685898-1685909 |
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| Autor principal: | |
| Otros Autores: | , , , , , , , , , , , , , , , , |
| Publicado: |
Frontiers Media SA
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| Acceso en línea: | Citation/Abstract Full Text Full Text - PDF |
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| 001 | 3286012773 | ||
| 003 | UK-CbPIL | ||
| 022 | |a 2235-2988 | ||
| 024 | 7 | |a 10.3389/fcimb.2025.1685898 |2 doi | |
| 035 | |a 3286012773 | ||
| 045 | 2 | |b d20251201 |b d20251231 | |
| 100 | 1 | |a Gatti, Giulia |u 1 Department of Medical and Surgical Sciences-DIMEC, Alma Mater Studiorum, University of Bologna, Bologna, Italy | |
| 245 | 1 | |a NGS-based approach for diagnostically unidentified Mycobacterium saskatchewanense, a rare non-tuberculous mycobacterium | |
| 260 | |b Frontiers Media SA |c Dec 2025 | ||
| 513 | |a Journal Article | ||
| 520 | 3 | |a IntroductionThe implementation of advanced technologies and algorithms for diagnosis and genome analysis has made a fundamental contribution to pathogens’ identification and investigation.MethodsThe study of non-tuberculous mycobacteria (NTM) benefited from a next-generation sequencing (NGS) approach, making it possible to describe sequences of rare pathogens. This study identified 20 diagnostically unknown isolates as Mycobacterium saskatchewanense ST 691, an environmental NTM. The isolates were sequenced on two different platforms to compare their throughput and to investigate shared and unique single nucleotide polymorphism (SNP) counts, phylogeny based on concatenated 16S, hsp65, and rpoB genes, and core-genome multilocus sequence typing (MLST), in order to broaden the current knowledge of Mycobacterium saskatchewanense .ResultsPrincipal component analysis on the three genes combined with the mutations’ annotation suggests that rpoB may serve as a suitable marker to distinguish M. saskatchewanense from other NTM.DiscussionOur results show that frontier studies performed using NGS can help in overcoming the limits of traditional diagnostic assays and deepen the knowledge on rare and uncommon NTM that are raising clinical concern. | |
| 610 | 4 | |a Illumina Inc | |
| 651 | 4 | |a United States--US | |
| 651 | 4 | |a Germany | |
| 653 | |a Infections | ||
| 653 | |a Software | ||
| 653 | |a RpoB protein | ||
| 653 | |a Pathogens | ||
| 653 | |a Phylogenetics | ||
| 653 | |a Heat shock proteins | ||
| 653 | |a Identification | ||
| 653 | |a Principal components analysis | ||
| 653 | |a Mutation | ||
| 653 | |a Leprosy | ||
| 653 | |a Nucleotide sequence | ||
| 653 | |a Genes | ||
| 653 | |a Genotype & phenotype | ||
| 653 | |a Drug resistance | ||
| 653 | |a Hsp65 protein | ||
| 653 | |a Next-generation sequencing | ||
| 653 | |a Tuberculosis | ||
| 653 | |a Maximum likelihood method | ||
| 653 | |a Whole genome sequencing | ||
| 653 | |a Genomic analysis | ||
| 653 | |a Single-nucleotide polymorphism | ||
| 653 | |a Multilocus sequence typing | ||
| 653 | |a Mycobacterium | ||
| 700 | 1 | |a Ingletto, Ludovica |u 1 Department of Medical and Surgical Sciences-DIMEC, Alma Mater Studiorum, University of Bologna, Bologna, Italy | |
| 700 | 1 | |a Dirani, Giorgio |u 2 Operative Unit of Microbiology, The Greater Romagna Hub Laboratory, Cesena, Italy | |
| 700 | 1 | |a Zannoli, Silvia |u 2 Operative Unit of Microbiology, The Greater Romagna Hub Laboratory, Cesena, Italy | |
| 700 | 1 | |a Taddei, Francesca |u 2 Operative Unit of Microbiology, The Greater Romagna Hub Laboratory, Cesena, Italy | |
| 700 | 1 | |a Colosimo, Claudia |u 1 Department of Medical and Surgical Sciences-DIMEC, Alma Mater Studiorum, University of Bologna, Bologna, Italy | |
| 700 | 1 | |a Dionisi, Laura |u 1 Department of Medical and Surgical Sciences-DIMEC, Alma Mater Studiorum, University of Bologna, Bologna, Italy | |
| 700 | 1 | |a Marzucco, Anna |u 2 Operative Unit of Microbiology, The Greater Romagna Hub Laboratory, Cesena, Italy | |
| 700 | 1 | |a Montanari, Maria Sofia |u 2 Operative Unit of Microbiology, The Greater Romagna Hub Laboratory, Cesena, Italy | |
| 700 | 1 | |a Denicolò, Agnese |u 2 Operative Unit of Microbiology, The Greater Romagna Hub Laboratory, Cesena, Italy | |
| 700 | 1 | |a Congestrì, Francesco |u 2 Operative Unit of Microbiology, The Greater Romagna Hub Laboratory, Cesena, Italy | |
| 700 | 1 | |a Grumiro, Laura |u 2 Operative Unit of Microbiology, The Greater Romagna Hub Laboratory, Cesena, Italy | |
| 700 | 1 | |a Brandolini, Martina |u 1 Department of Medical and Surgical Sciences-DIMEC, Alma Mater Studiorum, University of Bologna, Bologna, Italy | |
| 700 | 1 | |a Guerra, Massimiliano |u 2 Operative Unit of Microbiology, The Greater Romagna Hub Laboratory, Cesena, Italy | |
| 700 | 1 | |a Alessandra Mistral De Pascali |u 1 Department of Medical and Surgical Sciences-DIMEC, Alma Mater Studiorum, University of Bologna, Bologna, Italy | |
| 700 | 1 | |a Scagliarini, Alessandra |u 1 Department of Medical and Surgical Sciences-DIMEC, Alma Mater Studiorum, University of Bologna, Bologna, Italy | |
| 700 | 1 | |a Cricca, Monica |u 1 Department of Medical and Surgical Sciences-DIMEC, Alma Mater Studiorum, University of Bologna, Bologna, Italy, 2 Operative Unit of Microbiology, The Greater Romagna Hub Laboratory, Cesena, Italy | |
| 700 | 1 | |a Sambri, Vittorio |u 1 Department of Medical and Surgical Sciences-DIMEC, Alma Mater Studiorum, University of Bologna, Bologna, Italy, 2 Operative Unit of Microbiology, The Greater Romagna Hub Laboratory, Cesena, Italy | |
| 773 | 0 | |t Frontiers in Cellular and Infection Microbiology |g vol. 15 (Dec 2025), p. 1685898-1685909 | |
| 786 | 0 | |d ProQuest |t Public Health Database | |
| 856 | 4 | 1 | |3 Citation/Abstract |u https://www.proquest.com/docview/3286012773/abstract/embedded/75I98GEZK8WCJMPQ?source=fedsrch |
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| 856 | 4 | 0 | |3 Full Text - PDF |u https://www.proquest.com/docview/3286012773/fulltextPDF/embedded/75I98GEZK8WCJMPQ?source=fedsrch |