Repurposing FDA-Approved Drugs as Hendra Virus RNA-Dependent RNA Polymerase Inhibitors: A Comprehensive Computational Drug Discovery Approach

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Viruses vol. 17, no. 12 (2025), p. 1613-1633
Prif Awdur: Lalu, Anjana C
Awduron Eraill: Kundil, Varun Thachan, Joseph Bristow Ben, Dev Radul R., Amritha, Thaikkad, Subair Suhail, Raju Rajesh, Abhithaj, Jayanandan
Cyhoeddwyd:
MDPI AG
Pynciau:
Mynediad Ar-lein:Citation/Abstract
Full Text + Graphics
Full Text - PDF
Tagiau: Ychwanegu Tag
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MARC

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024 7 |a 10.3390/v17121613  |2 doi 
035 |a 3286359097 
045 2 |b d20250101  |b d20251231 
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100 1 |a Lalu, Anjana C 
245 1 |a Repurposing FDA-Approved Drugs as Hendra Virus RNA-Dependent RNA Polymerase Inhibitors: A Comprehensive Computational Drug Discovery Approach 
260 |b MDPI AG  |c 2025 
513 |a Journal Article 
520 3 |a Hendra virus (HeV) is a highly pathogenic zoonotic paramyxovirus that poses a serious threat to human and equine health, yet no approved antivirals or vaccines currently exist. RNA-dependent RNA polymerase (RdRp) of Hendra virus represents a critical and attractive target for antiviral drug development, given its essential role in both viral genome replication and mRNA transcription. Due to the lack of a human homolog, it is more druggable and less likely to cause host toxicity. Its sequence conservation among related paramyxoviruses further highlights its potential for the development of broad-spectrum inhibitors. This study offers the first comprehensive computational analysis of the Hendra virus RdRp, potentially promising FDA-approved drugs as possible inhibitors. A homology model of RdRp was generated in the absence of experimental three-dimensional (3D) structure, followed by virtual screening and molecular dynamics (MD) simulations to evaluate the drug binding and stability. Based on the highest energy, four FDA-approved drugs selected were menadiol diphosphate (−49.88 kcal/mol), masoprocol (−39.69 kcal/mol), pamidronic acid (−34.29 kcal/mol), and dinoprostone (−46.90 kcal/mol). Furthermore, these compounds exhibited significant interactions with the catalytic GDNE motif. With strong conformational stability and pharmacokinetic profile, masoprocol and menadiol diphosphate showed the most stable and energetically favorable interactions within the RdRp active site. These findings suggest their potential as repurposed therapeutic candidates against Hendra virus infection and they provide a structural basis for the development of broad-spectrum paramyxovirus inhibitors, justifying additional experimental confirmation. 
610 4 |a Food & Drug Administration--FDA 
651 4 |a Australia 
653 |a Physiology 
653 |a RNA polymerase 
653 |a RNA-directed RNA polymerase 
653 |a Toxicity 
653 |a Binding sites 
653 |a Hydrogen 
653 |a Drug development 
653 |a Computer applications 
653 |a DNA-directed RNA polymerase 
653 |a Antiviral agents 
653 |a Bioavailability 
653 |a Proteins 
653 |a Crystal structure 
653 |a Simulation 
653 |a Fatalities 
653 |a RNA viruses 
653 |a Pharmacokinetics 
653 |a FDA approval 
653 |a Pamidronic acid 
653 |a Conserved sequence 
653 |a Bisphosphonates 
653 |a Ligands 
653 |a Viruses 
653 |a Homology 
700 1 |a Kundil, Varun Thachan 
700 1 |a Joseph Bristow Ben 
700 1 |a Dev Radul R. 
700 1 |a Amritha, Thaikkad 
700 1 |a Subair Suhail 
700 1 |a Raju Rajesh 
700 1 |a Abhithaj, Jayanandan 
773 0 |t Viruses  |g vol. 17, no. 12 (2025), p. 1613-1633 
786 0 |d ProQuest  |t Health & Medical Collection 
856 4 1 |3 Citation/Abstract  |u https://www.proquest.com/docview/3286359097/abstract/embedded/L8HZQI7Z43R0LA5T?source=fedsrch 
856 4 0 |3 Full Text + Graphics  |u https://www.proquest.com/docview/3286359097/fulltextwithgraphics/embedded/L8HZQI7Z43R0LA5T?source=fedsrch 
856 4 0 |3 Full Text - PDF  |u https://www.proquest.com/docview/3286359097/fulltextPDF/embedded/L8HZQI7Z43R0LA5T?source=fedsrch