Divicine induces endothelial cells injury and its potential mechanism

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書誌詳細
出版年:	Biocell vol. 46, no. 7 (2022), p. 1725
第一著者:	Long, Su
その他の著者:	Lin, Zhexuan, Li, Hui, Luo, Hongjun, Luo, Wenhong
出版事項:	Tech Science Press
主題:	Reactive oxygen species Transmission electron microscopy Cell death Deferoxamine Glutathione Umbilical vein Vascular system β-Glucosidase Cell viability Mitochondria Iron Endothelial cells Beans Malaria Oxidation Microscopy Cardiovascular disease Enzymes Dehydrogenases Cell culture Ferroptosis
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その他の書誌記述
抄録:	Divicine is an active pyrimidine aglycone, generated from vicine by the enzyme β-glucosidase upon ingestion of fava beans. In this study, we investigated the effect of divicine on cultured human umbilical vein endothelial cells (HUVECs) and explored the potential mechanisms. Incubation HUVECs with 18.5–85.1 μM divicine resulted in a concentration and time dependent decrease of cell viability, followed by decrease of cellular reduced glutathione, as well as increase of reactive oxygen species (ROS), malondialdehyde (MDA), and labile iron pool. Transmission electron microscopy confirmed that the divicine treated HUVECs’ mitochondria had shrunk. Importantly, the administration of desferrioxamine, an iron chelator, to the divicine treated HUVECs significantly reduced iron overload and cell death and decreased cellular ROS and MDA. These results demonstrated that divicine could cause damaging of endothelial cells, and ferroptosis might be involved in divicine induced HUVECs injury, reminding long term ingestion of fava beans might be harmful to vascular system, especially for those suffering from glucose 6-phosphate dehydrogenase deficiency.
ISSN:	0327-9545 0300-3426 0326-3142
DOI:	10.32604/biocell.2022.018508
ソース:	Biological Science Database