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Divicine induces endothelial cells injury and its potential mechanism

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Pubblicato in:Biocell vol. 46, no. 7 (2022), p. 1725
Autore principale: Long, Su
Altri autori: Lin, Zhexuan, Li, Hui, Luo, Hongjun, Luo, Wenhong
Pubblicazione:
Tech Science Press
Soggetti:
Reactive oxygen species
Transmission electron microscopy
Cell death
Deferoxamine
Glutathione
Umbilical vein
Vascular system
β-Glucosidase
Cell viability
Mitochondria
Iron
Endothelial cells
Beans
Malaria
Oxidation
Microscopy
Cardiovascular disease
Enzymes
Dehydrogenases
Cell culture
Ferroptosis
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Abstract:Divicine is an active pyrimidine aglycone, generated from vicine by the enzyme β-glucosidase upon ingestion of fava beans. In this study, we investigated the effect of divicine on cultured human umbilical vein endothelial cells (HUVECs) and explored the potential mechanisms. Incubation HUVECs with 18.5–85.1 μM divicine resulted in a concentration and time dependent decrease of cell viability, followed by decrease of cellular reduced glutathione, as well as increase of reactive oxygen species (ROS), malondialdehyde (MDA), and labile iron pool. Transmission electron microscopy confirmed that the divicine treated HUVECs’ mitochondria had shrunk. Importantly, the administration of desferrioxamine, an iron chelator, to the divicine treated HUVECs significantly reduced iron overload and cell death and decreased cellular ROS and MDA. These results demonstrated that divicine could cause damaging of endothelial cells, and ferroptosis might be involved in divicine induced HUVECs injury, reminding long term ingestion of fava beans might be harmful to vascular system, especially for those suffering from glucose 6-phosphate dehydrogenase deficiency.
ISSN:0327-9545
0300-3426
0326-3142
DOI:10.32604/biocell.2022.018508
Fonte:Biological Science Database