Profiling gene alterations in striatonigral neurons associated with incubation of methamphetamine craving by cholera toxin subunit B-based fluorescence-activated cell sorting

Uloženo v:

Podrobná bibliografie
Vydáno v:	Frontiers in Cellular Neuroscience (Feb 12, 2025)
Hlavní autor:	Altshuler, Rachel D
Další autoři:	Burke, Megan A M, Garcia, Kristine T, Class, Kenneth, Cimbro, Raffaello, Li, Xuan
Vydáno:	Frontiers Research Foundation
Témata:	University of Maryland United States > US Behavior Cholera toxin Catheters Caudate-putamen Molecular modelling Spiny neurons Flow cytometry Genes Neostriatum Laboratory animals Narcotics Proteins Fluorescence Abstinence Polyethylene Dopamine Surgery FDA approval Experiments Methamphetamine Cholera Enzymes Toxins Roles Neurosciences
On-line přístup:	Citation/Abstract Full Text + Graphics Full Text - PDF
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!

Popis
Abstrakt:	Introduction: In both rats and humans, methamphetamine (Meth) seeking progressively increases during abstinence, a behavioral phenomenon termed “incubation of Meth craving”. We previously demonstrated a critical role of dorsal striatum (DS) in this incubation in rats. However, circuit-specific molecular mechanisms in DS underlying this incubation are largely unknown. Here we combined a newly developed fluorescence-activated sorting (FACS) protocol with fluorescence-conjugated cholera toxin subunit B-647 (CTb-647, a retrograde tracer) to examine gene alterations in the direct-pathway (striatonigral) medium spiny neurons (MSNs) associated with incubation of Meth craving.
ISSN:	1662-5102
DOI:	10.3389/fncel.2025.1542508
Zdroj:	Science Database