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DNA Barcoding for Tracing Biodiversity in Mixed Crop Food Products: A Proof of Concept Within the BioValue Project

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Publicado en:Foods vol. 14, no. 18 (2025), p. 3256-3271
Autor principal: Maria-Dimitra, Tsolakidou
Otros Autores: Nikoloudakis Nikolaos, Tisseyre Cyril, Knez Marija, Barilli Eleonora, Mattas Konstadinos, Katsiotis Andreas
Publicado:
MDPI AG
Materias:
Food security
DNA sequencing
Authenticity
Labels
Agricultural production
Food products
Biological materials
Domestication
Ingredients
Identification
Materials traceability
Biodiversity
Mixed cropping
Food supply
Crops
Raw materials
Ribosomal DNA
Food processing
Genetic diversity
Climate change
Genetic testing
Food
Processed foods
Gene sequencing
Plant-based foods
DNA barcoding
Descriptive labeling
Deoxyribonucleic acid > DNA
Supply chains
Sustainable food systems
Food safety
Food contamination
Morphology
Agrobiodiversity
Legumes
Acceso en línea:Citation/Abstract
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Resumen:In a world of rapidly globalizing food markets, biodiversity, authenticity, and the safety of food products have become a universal concern. DNA barcoding is a widely used molecular-based method that can identify biological material and is used for the traceability of both raw materials and ingredients in processed food. In the present study, contacted within the framework of the BioValue Horizon Project, which promotes the role of agrobiodiversity in sustainable food systems, DNA barcoding using the ITS and rbcL markers was employed as a proof-of-concept approach to reveal the biodiversity and authenticity of ten commercial plant-based products. Following successful DNA amplification and sequencing using six products as a proof-of-concept, a diverse range of plant genera and species were identified, verifying biodiversity. A strong correlation between ITS and rbcL-based markers was demonstrated, supporting their combined use for reliable species-level biodiversity assessment. Finally, heat map analysis of label contents and sequencing-based genera identification confirmed high concordance between label claims and sequencing results in most cases, though undeclared species and absent labeled taxa were also detected, highlighting potential mislabeling or cross-contamination.
ISSN:2304-8158
DOI:10.3390/foods14183256
Fuente:Agriculture Science Database