DNA Barcoding for Tracing Biodiversity in Mixed Crop Food Products: A Proof of Concept Within the BioValue Project

שמור ב:
מידע ביבליוגרפי
הוצא לאור ב:Foods vol. 14, no. 18 (2025), p. 3256-3271
מחבר ראשי: Maria-Dimitra, Tsolakidou
מחברים אחרים: Nikoloudakis Nikolaos, Tisseyre Cyril, Knez Marija, Barilli Eleonora, Mattas Konstadinos, Katsiotis Andreas
יצא לאור:
MDPI AG
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גישה מקוונת:Citation/Abstract
Full Text + Graphics
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024 7 |a 10.3390/foods14183256  |2 doi 
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045 2 |b d20250915  |b d20250930 
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100 1 |a Maria-Dimitra, Tsolakidou  |u Department of Agricultural Sciences, Biotechnology and Food Science, Cyprus University of Technology, 3603 Limassol, Cyprus; maria.tsolakidou@cut.ac.cy 
245 1 |a DNA Barcoding for Tracing Biodiversity in Mixed Crop Food Products: A Proof of Concept Within the BioValue Project 
260 |b MDPI AG  |c 2025 
513 |a Journal Article 
520 3 |a In a world of rapidly globalizing food markets, biodiversity, authenticity, and the safety of food products have become a universal concern. DNA barcoding is a widely used molecular-based method that can identify biological material and is used for the traceability of both raw materials and ingredients in processed food. In the present study, contacted within the framework of the BioValue Horizon Project, which promotes the role of agrobiodiversity in sustainable food systems, DNA barcoding using the ITS and rbcL markers was employed as a proof-of-concept approach to reveal the biodiversity and authenticity of ten commercial plant-based products. Following successful DNA amplification and sequencing using six products as a proof-of-concept, a diverse range of plant genera and species were identified, verifying biodiversity. A strong correlation between ITS and rbcL-based markers was demonstrated, supporting their combined use for reliable species-level biodiversity assessment. Finally, heat map analysis of label contents and sequencing-based genera identification confirmed high concordance between label claims and sequencing results in most cases, though undeclared species and absent labeled taxa were also detected, highlighting potential mislabeling or cross-contamination. 
653 |a Food security 
653 |a DNA sequencing 
653 |a Authenticity 
653 |a Labels 
653 |a Agricultural production 
653 |a Food products 
653 |a Biological materials 
653 |a Domestication 
653 |a Ingredients 
653 |a Identification 
653 |a Materials traceability 
653 |a Biodiversity 
653 |a Mixed cropping 
653 |a Food supply 
653 |a Crops 
653 |a Raw materials 
653 |a Ribosomal DNA 
653 |a Food processing 
653 |a Genetic diversity 
653 |a Climate change 
653 |a Genetic testing 
653 |a Food 
653 |a Processed foods 
653 |a Gene sequencing 
653 |a Plant-based foods 
653 |a DNA barcoding 
653 |a Descriptive labeling 
653 |a Deoxyribonucleic acid--DNA 
653 |a Supply chains 
653 |a Sustainable food systems 
653 |a Food safety 
653 |a Food contamination 
653 |a Morphology 
653 |a Agrobiodiversity 
653 |a Legumes 
700 1 |a Nikoloudakis Nikolaos  |u Department of Agricultural Sciences, Biotechnology and Food Science, Cyprus University of Technology, 3603 Limassol, Cyprus; maria.tsolakidou@cut.ac.cy 
700 1 |a Tisseyre Cyril  |u ECOZEPT France SAS, Le Barcelone, Bât. 12, 145 Rue Guillaume Janvier, FR-34070 Montpellier, France; tisseyre@ecozept.com 
700 1 |a Knez Marija  |u Capacity Development in Nutrition, 11000 Belgrade, Serbia; marijaknez186@gmail.com 
700 1 |a Barilli Eleonora  |u Solintagro SL, Calle Escritora Rosa Chacel 4 Local 2, 14004 Cordoba, Spain; e.nora@libero.it 
700 1 |a Mattas Konstadinos  |u Department of Agricultural Economics, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece; mattas@auth.gr 
700 1 |a Katsiotis Andreas  |u Department of Agricultural Sciences, Biotechnology and Food Science, Cyprus University of Technology, 3603 Limassol, Cyprus; maria.tsolakidou@cut.ac.cy 
773 0 |t Foods  |g vol. 14, no. 18 (2025), p. 3256-3271 
786 0 |d ProQuest  |t Agriculture Science Database 
856 4 1 |3 Citation/Abstract  |u https://www.proquest.com/docview/3254516222/abstract/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch 
856 4 0 |3 Full Text + Graphics  |u https://www.proquest.com/docview/3254516222/fulltextwithgraphics/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch 
856 4 0 |3 Full Text - PDF  |u https://www.proquest.com/docview/3254516222/fulltextPDF/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch