Lipidomic Characterization of Muscle and Head of Litopenaeus vannamei Exposed to Lactococcus lactis D1813 at Varied Levels of Salinity and Dissolved Oxygen

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Publicat a:Food Science & Nutrition vol. 13, no. 11 (Nov 1, 2025)
Autor principal: Adil, Muhammad
Altres autors: Xiao, Xinglong, Waseem, Muhammad, Afraz, Muhammad Talha, Javed, Muhammad Rizwan, Alohali, Basim M., Mohamed Ahmed, Isam A., Manzoor, Muhammad Faisal, Madilo, Felix Kwashie
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John Wiley & Sons, Inc.
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Accés en línia:Citation/Abstract
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022 |a 2048-7177 
024 7 |a 10.1002/fsn3.71141  |2 doi 
035 |a 3266605420 
045 0 |b d20251101 
084 |a 233132  |2 nlm 
100 1 |a Adil, Muhammad  |u School of Food Science and Engineering, South China University of Technology, Guangzhou, China 
245 1 |a Lipidomic Characterization of Muscle and Head of Litopenaeus vannamei Exposed to Lactococcus lactis D1813 at Varied Levels of Salinity and Dissolved Oxygen 
260 |b John Wiley & Sons, Inc.  |c Nov 1, 2025 
513 |a Journal Article 
520 3 |a ABSTRACT The present study examined the impacts of Lactococcus lactis D1813, salinity (8 and 25 ppt), and dissolved oxygen (DO) concentrations of 8.5 and 3.5 mg/L on Litopenaeus vannamei muscle and head lipidomic profile. The muscle and head were examined using liquid chromatography‐mass spectrometry (LC–MS/MS) for lipidomic nutrient profiling. The lipidomic profiling revealed the primary nutritional metabolites in L. vannamei muscle and head, which are composed of glycerophospholipids, sphingolipids, sterols, and saturated fatty acids. Huang head had the most glycerophospholipids and sterols, including PC (12:0/18:2), PC (18:0/20:4), PC (16:1/22:6), PC (10:1e/20:4), PC (33:0/18:2), Cer (d14:1/20:0), SM (t18:1/22:4), and SM (d18:0/16:1). Conversely, the T3BS head group had a much greater proportion of sphingolipids and saturated fatty acids, including Cer (d14:1/20:0), SM (t18:1/22:4), SM (d18:0/16:1), SM (d18:1/18:3), and butyric acid, stearic acid, myristic acid, arachidic acid, and lauric acid (p < 0.05). The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated the significant enrichment of glycerophospholipid metabolism, sphingolipid metabolism, glycerolipid metabolism, linolenic acid metabolism, and alpha‐linolenic acid metabolism in T3BS muscle and head. The findings unequivocally demonstrate that the supplement of L. lactis D1813 under 25 ppt salinity and 3.5 mg/L DO levels significantly raises the content of sphingolipids and saturated fatty acids and enriches the metabolic pathways of linolenic acid and alpha‐linolenic acid in L. vannamei. The results indicated that L. lactis D1813 could be used as an effective probiotic to enhance shrimp tolerance and nutritional quality in aquaculture systems under environmental stress conditions. Also, a more desirable lipid profile renders them beneficial to human health, particularly cardiovascular health and brain function. Additional research can be done to assess the therapeutic advantages of L. vannamei‐derived lipids in humans regarding cardiovascular health, cancer, and brain functioning. 
651 4 |a United States--US 
651 4 |a China 
653 |a Mass spectrometry 
653 |a Lauric acid 
653 |a Liquid chromatography 
653 |a Butyric acid 
653 |a Probiotics 
653 |a Muscles 
653 |a Salinity effects 
653 |a Stearic acid 
653 |a Dissolved oxygen 
653 |a Sterols 
653 |a Brain 
653 |a Metabolites 
653 |a Metabolism 
653 |a Oxidative stress 
653 |a Water quality 
653 |a Lipids 
653 |a Linolenic acid 
653 |a Fatty acids 
653 |a Metabolic pathways 
653 |a Lipid metabolism 
653 |a Mass spectroscopy 
653 |a Environmental stress 
653 |a Aquaculture 
653 |a Sphingolipids 
653 |a Salinity 
653 |a Reagents 
653 |a Polyunsaturated fatty acids 
653 |a Environmental conditions 
653 |a Caustic soda 
653 |a Signal transduction 
653 |a Nutritive value 
653 |a Hydrochloric acid 
653 |a Phospholipids 
653 |a Litopenaeus vannamei 
653 |a Lactococcus lactis 
653 |a Environmental 
700 1 |a Xiao, Xinglong  |u School of Food Science and Engineering, South China University of Technology, Guangzhou, China 
700 1 |a Waseem, Muhammad  |u Department of Food Science &amp;amp; Technology, Faculty of Agriculture &amp;amp; Environment, The Islamia University of Bahawalpur, Punjab, Pakistan 
700 1 |a Afraz, Muhammad Talha  |u School of Food Science and Engineering, South China University of Technology, Guangzhou, China 
700 1 |a Javed, Muhammad Rizwan  |u Department of Food Science &amp;amp; Technology, Faculty of Agriculture &amp;amp; Environment, The Islamia University of Bahawalpur, Punjab, Pakistan 
700 1 |a Alohali, Basim M.  |u Department of Food Science and Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh, Saudi Arabia 
700 1 |a Mohamed Ahmed, Isam A.  |u Department of Food Science and Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh, Saudi Arabia 
700 1 |a Manzoor, Muhammad Faisal  |u School of Food Science and Engineering, South China University of Technology, Guangzhou, China 
700 1 |a Madilo, Felix Kwashie  |u Food Science and Technology Department, Ho Technical University, Ho, Ghana 
773 0 |t Food Science & Nutrition  |g vol. 13, no. 11 (Nov 1, 2025) 
786 0 |d ProQuest  |t Health & Medical Collection 
856 4 1 |3 Citation/Abstract  |u https://www.proquest.com/docview/3266605420/abstract/embedded/Q8Z64E4HU3OH5N8U?source=fedsrch 
856 4 0 |3 Full Text  |u https://www.proquest.com/docview/3266605420/fulltext/embedded/Q8Z64E4HU3OH5N8U?source=fedsrch 
856 4 0 |3 Full Text - PDF  |u https://www.proquest.com/docview/3266605420/fulltextPDF/embedded/Q8Z64E4HU3OH5N8U?source=fedsrch